Freeze/Boil Cycling for Cell Lysis for rep-PCR

Lemuel S. del Rosario ldelrosario at laba.tdh.state.tx.us
Fri Feb 16 13:22:15 EST 1996


I am attempting to perform rep-PCR on gram-negative enteric bacteria for 
molecular subtyping purposes (Versalovic, et.al).The method I am using to 
liberate the DNA from whole cells is to do freeze/thaw cycles. Can anyone 
tell me specifics on how to do this crude DNA extraction, such as how 
many cycles are usual needed and how long to boil/freeze the samples.

Thanks,

Lemuel

Texas Department of Health
Bureau of Laboratories
Microbiological Investigation Section
ldelrosario at laba.tdh.state.tx.us



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