Antibody dimer/monomer

MV. Hayes bimvh at
Sat Feb 17 08:28:24 EST 1996

Did you use fresh mercaptoethanol? 

Jose F Gutierrez Marcos (jfgm at wrote:

: I have specific antibodies for a 51kD protein but after running the total 
: protein extract on a Laemli acrylamide gel and do the western blot I got 
: two bands: a faint one of around 51kD and a strong one of around 100kD.

: Does anyone knows how to explain that result on a denaturing gel.

: Thanks

: Jose

: Please mail to: jfgm at

: >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>


Dr Michelle Hayes
Department of Biochemistry
University of Bristol

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