5' biotinylation of RNA

Tom Vink tvink at xs4all.nl
Sat Feb 17 20:45:30 EST 1996


Does somebody has a good protocol to label in vitro transcribed RNA at
the 5' end with biotin. The only thing I could think of is ligating a
biotinylated oligo to the end with T4 RNA ligase. Would it have to be
a RNA oligo ?
Tom Vink
Department of Haematology
University Hospital Utrecht
The Netherlands




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