Can DTT be substituted by DTE ????
minetti at sage.cc.purdue.edu
Sun Feb 18 22:51:55 EST 1996
In article <4g1v04$53q at merkurius.lu.se>, <janke> wrote:
>Does any body know what the difference is in using DTT
>or DTE? Are they similar enough to be substituted
>in most reactions? If not, why?
>Any help is welcome.
>Please mail your answer also to my address, so
>that I do not miss it. Thanx
>janke at lykos.gen.lu.se
The commonly used Cleland's reagent is the racemate 1,4-dithio-DL-threitol:
H OH HO H
| | | |
| | | |
HO H H OH
DTE is the _meso_ compound, with no optical activity, 1,4-dithio-erythritol:
I think that they have the same effectiveness in keeping the sulfhydrile
groups of the protein in the reduced state, but did not carry out any
detailed study. I have compared their properties as substrates in the
enzymatic reduction of methionine-sulfoxide, and found no differences.
But that was a very special situation.
I remember that the 1,4-dithio-L-threitol is available, but have no idea as
to whether it is required for special purposes.
BTW, consider the difference in the price!
Giampaolo Minetti ~.:~
Purdue University \:/
Department of Chemistry /:\
327 Wetherill Building /:.~\
West Lafayette IN-47906 /-~_~_\
Tel. 317-494-5275 (_______)
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