Maleic acid buffer: Help me make it
rosanne at dance.tap.csiro.au
Sun Feb 18 22:46:52 EST 1996
kernan at uga.cc.uga.edu (Rich Kernan) wrote:
>In article <scj-1502960907510001 at jmm3.ahabs.wisc.edu>,
>scj at calshp.cals.wisc.edu (Scott C. Johnson) wrote:
>>That there were problems making up a 10x stock of buffer 1 i.e. 1M maleic acid, 1.5M NaCl, pH 7.5.
We routinely make up a 10x stock of this buffer. This is our recipe:
To make 1 litre:
Maleic acid: 116.1g
Water to about 800ml
At this point the maleic acid is completely insoluble. Start adding NaOH pellets while stirring the solution. Have patience, the ppt will not solubilize until the pH reaches about 5.5 to 6.0. At this point, start adding the pellets VERY carefully as it is easy for the pH to overshoot. When the pH reaches 7.5, top up with water to the correct volume and then autoclave if desired. This works every time.
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