Double Labeling Problem

Stephanie Dillon srd9025 at garnet.acns.fsu.edu.
Sun Feb 18 21:49:37 EST 1996


I am double labeling a protein grown in E. coli.  I am labeling with 15N 
and 13C.  The 15N label is working fine by itself. I get a good amount 
of protein, but when I tried to double label the growth basically died.  
I am introducing the 13C through glucose.  Has anyone else had this 
problem?  Suggestions of possible problems would be welcome.





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