Smiles in agarose gels
landryp at ERE.UMontreal.CA
Mon Feb 19 20:42:56 EST 1996
In order to separate DNA fragments obtained from RAPD, I make the
amplification samples run through an horizontal agarose gel.
Unfortunately, I always get this "smile" that bends all the bands and
makes the gel analysis much more complicated. The gels are 1,5%, and I've
tried different voltages from 20 to 80 volts, and although I noticed that
the "smile" is reduced with decreasing voltages, it isn't clear enough to
allow a perfect analysis. I'd be glad to hear any suggestion, advice or
magic trick to improve the situation.
Thanks in advance.
Dept. des Sciences Biologiques
Universite de Montreal
C.P. 6128, succursale centre ville
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