Exponentially growing U937 ??

Stephen R. Lasky Stephen_Lasky at brown.edu
Mon Feb 19 08:47:22 EST 1996


In article <4fsclh$cbc at news.fhg.de>, seegert at ita.fhg.de (Dirk Seegert) wrote:


Dirk:  Its not really clear what you want to do.  We don't ususally let
our myeloid cells grow to densities higher than 1 million per ml, although
they will still prolferate at higher densities.  We usually split our
myeloid leukemia cells (we're not working with U937 right now, but we have
in the past) down to 60,000 to 100,000 per ml, let them double till they
get to 800,000 to 1 million per ml and then split them back.  This keeps
them healthy and growing continuosly.  From my experience, unless you have
a particular reason to want the cells to arrest at high densities, its
better to not let them grow to their maximum density.  If you are trying
to synchronize your cells, in our hands high density arrest doesn't give
very good synchronization of suspension culture cells.

SRLasky

> Hi Netters,
> 
> is anybody out there who could give me some rules to get exponentially
> growing U937, i.e. by diluting the cells xy hours prior to harvest to
> a special concentration?!
> 
> Thanks in advance
> 
> Dirk Seegert, Ph.D.
> Fraunhofer Institute Hannover
> Germany

-- 
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Stephen R. Lasky Ph.D.   Brown U/Roger Williams Medical Center,  Providence, RI.   
Phone: 401-456-5672     Fax: 401-456-6569     e:mail: Stephen_Lasky at brown.edu
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America may be unique in being a country which has leapt from barbarism to decadence without touching civilization.  John O'Hara.
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