Using Biotin in ELISA's
bijgh at zeus.bris.ac.uk
Mon Feb 19 09:28:40 EST 1996
Karl Eisele (cbiei01 at commlink.zdv.uni-tuebingen.de) wrote:
: I'd say we need a bit more information on your setup to help you.
: However, one mistake which is frequently made is blocking your ELISA
: with dried milk which itself contains biotin (!!) --> high background (or
: unspecific binding).
I read that somewhere and have been using BSA or gelatin as a blocking
agent. The problem I'm having isn't a high background as such (empty
wells give negative results) but false positive binding to any protein I
coat the plate with.
Thanks for your interest!
Jared Head at the Department of Biochemistry, University of Bristol
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