Using Biotin in ELISA's

[Karl Eisele]

Hi,

I'd say we need a bit more information on your setup to help you. 
However, one mistake which is frequently made is blocking your ELISA 
with dried milk which itself contains biotin (!!) --> high background (or 
unspecific binding).

Hope I could help, otherwise drop me a line.

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Harold V. Taylor
Physiol. Chem. Institut
Universtaet Tuebingen, Germany
cbiei01 at mailserv.zdv.uni-tuebingen.de
phone: 7071-29-3353
fax:   7071-29-3361
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