Introduction of stop codons

J. Kaufmann kaufmann at
Tue Feb 20 06:47:17 EST 1996


I was thinking of introducing stop codons in the sequence of my protein,
instead of deleting different lenghts of the C-terminal part of this
protein (because I can perform site directed mutagenesis easily but I am
scared to spend too much time setting up the PCR conditions for the
deletions I want to make).

Did anybody try that?
Did it work or not? Is there no problem for transcription or translation
to occur?

Thank you for answering by E-Mail.


Jocelyne Kaufmann
Laboratoires de Recherche Louis Jeantet
Centre Medical Universitaire
1, rue Michel Servet
CH-1211 Geneve 4
e-mail: kaufmann at

More information about the Methods mailing list