Smiles in agarose gels
Tue Feb 20 09:41:51 EST 1996
In article <landryp.824780576 at alize.ERE.UMontreal.CA>,
landryp at ERE.UMontreal.CA says...
> In order to separate DNA fragments obtained from RAPD, I make
>amplification samples run through an horizontal agarose gel.
>Unfortunately, I always get this "smile" that bends all the bands and
>makes the gel analysis much more complicated. The gels are 1,5%, and
>tried different voltages from 20 to 80 volts, and although I noticed
>the "smile" is reduced with decreasing voltages, it isn't clear enough
>allow a perfect analysis. I'd be glad to hear any suggestion, advice
>magic trick to improve the situation.
>Thanks in advance.
>Dept. des Sciences Biologiques
>Universite de Montreal
>C.P. 6128, succursale centre ville
>we had the same problem running RAPD reactions out on gels. Adding a
small amount of EtBr to our ficoll loading dye (1µl EtBr stock
(10mg/ml) per 1ml ficoll). I'm assumming that each band within each
lane is 'smiling' and its not just one big smile across the whole gel.
Hope this helps.
More information about the Methods