Maleic acid buffer: Help me make it

Jon jcowan at earthlink.net
Sat Feb 17 14:03:25 EST 1996


nikolaic at VISAR.WUSTL.EDU (Nikolai Chitaev) wrote:

>> From: scj at calshp.cals.wisc.edu (Scott C. Johnson)
>> Subject: Maleic acid buffer: Help me make it
>> Date: 15 Feb 1996 15:09:12 GMT
>> 
><snipp>
>> 
>> Am I an idiot? What am I missing? What are they doing differently?
>> 
>> Thanks,
>> Scott
>> 

>Hi, Scott.
>No! You a no more idiot than people at BM. You only miss one thing. They,
>i.e. people at BM, had never ever made 1M maleic acid buffer, pH 7.5.
>If you`ll read carefuly p80 of BM catalogue (1996) "Genius Wash and Block Buffer Set"
>you`ll see that products they sell are 10x solutions in respect to anything, but are
>1x solution in respect to maleic acid buffer. They even sell 1x buffer to dilute their
>10x stock solutions!

>So, do ahead, prepare all solution yourself using 0.1 M maleic acid, pH 7.5, but 10x with
>respect to other components, and then additionaly preparte a lot of 0.1 M maleic
>acid, pH 7.5 to dilute those 10x solutions.

>I can assure you that 0.1 M maleic acid solution with pH 7.5 could be easy prepared.
>I did it. Essentialy as described.

>Nikolai
>http://128.252.119.253

After calling B-M's tech support, I found out the same thing.  A 10 X
solution is 0.1M


Jon Cowan
Dept. of Biology
University of Alabama at Birmingham
jcowan at earthlink.net




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