Making cDNA library from Poly A minus RNA

john brennand john.brennand at
Wed Feb 21 06:37:30 EST 1996

Any of the kits using random hexamers (or make your own) should work 
fine - we used a Pharmacia kit with no bother.

You may need to do some 1st strand sighting experiments with P32 to get 
the primer:RNA ratio optimal for yield & product length


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