Expression vector containing SV40 large T antigen?

john brennand john.brennand at gbapr.zeneca.com
Thu Feb 22 11:17:51 EST 1996


Nigel

No there aren't.

What he (& we) are looking for is a vector that carries SV40 large Tag 
and not just the SV40 ori to which Tag binds to induce replication.

COS cells, for example were transfected to express large T and give high 
level expression from "loads" of vectors - but you are limited to these 
cells 

John





More information about the Methods mailing list