Partial RE digestion - please advise!
jpcd0 at mole.bio.cam.ac.uk
Thu Feb 22 07:55:25 EST 1996
In article <voellerj-2002961436470001 at 18.104.22.168>,
voellerj at gunet.georgetown.edu (Jim Voeller) wrote:
> In article <jpcd0-2002961403240001 at macr1-4.welc.cam.ac.uk>,
> jpcd0 at mole.bio.cam.ac.uk (John Dixon) wrote:
> >As BsmB1 is a hapaxoterministic enzyme you should be able to clone the
> Please define hapaxoterministic. Just curious, thanks.
Hapaxoterministic was coined by Shelby Berger, I think, in Anal Biochem,
222 1-8 1994, to describe enzymes that cut outside their recognition
sequence, giving unspecified overhang sequence.
BsmB1 for instance cuts CGTCTCN NNNNNNNN
So that the overhang at any one cut point is 1 of the 256 combinations of
ACGT. Apart from palindromic ones, these ends are non-self-complementary
and cannot concatemerize.
They can be quite useful occasionally.
John Dixon Lab 44 (1223) 334131
Wellcome/CRC Institute Fax 44 (1223) 334134
Department of Genetics
United Kingdom e-m: jpcd0 at mole.bio.cam.ac.uk
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