Smiles in agarose gels

Susan Jane Hogarth sjhogart at unity.ncsu.edu
Wed Feb 21 07:25:40 EST 1996


WSchick at aol.com wrote:

>Check your electrodes--are they full width of your cell?  short electrodes
>could concentrte current in the center of lyour gel to give you a smile.

Is this true? A grad student in my lab runs starch (protein) gels with a piece
of platinium wire just clipped into the buffer compartment - no more than 1
inch long. He doesn't seem to get any radical side to side variation on the
gel. Is it different with agarose (DNA) gels?
-- 


Susan Jane Hogarth
http://www4.ncsu.edu/unity/users/s/sjhogart/public/home.html




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