Qiaex problems

Dr. Arthur L. Kruckeberg bmbckh at biovax.leeds.ac.uk
Fri Feb 23 12:04:57 EST 1996


In article <4gipuf$rgd at bigblue.oit.unc.edu>
plc at med.unc.edu (Philip L. Carl) writes:

> >>We use Qiaex II from Qiagen to isolate DNA fragments from agarose for the
> >>purposes of ligation.  We have had LOTS of problems getting our ligations
> >>to work.  Has anybody else had similiar problems?  Has anybody figured out
> >>how to correct the problem.  I realize one correction would be to use a
> >>different method to isolate DNA from agarose.  Anybody have a favorite
> >>alternative?
> >>
> >>Thanks
> >>
> >>John Stebbins :

We've had similar problems. We overcome them by using ultrapure grade
agarose.

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Chris Hoyle

   Department of Biochemistry and Molecular Biology
University of Leeds             phone +44 0113 2333172
Leeds LS2 9JT                   FAX   +44 0113 2333167
Great Britain           e-mail bmbckh at biovax.leeds.ac.uk
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