Epitope Tagging Suggestions Wanted

Dr. Arthur L. Kruckeberg bmbckh at biovax.leeds.ac.uk
Fri Feb 23 12:01:26 EST 1996

In article <312CC8EB.2533 at phy164.physiology.uab.edu>
Wayne Duck <wayne-duck at phy164.physiology.uab.edu> writes:

> A. John Watson wrote:
> > 
> > I am planning to epitope tag one of my favorite receptors and am wondering
> > what systems have people found to be particularly reliable/unreliable with
> > respect to (a) choice of tag sequence and
> > (b) source of anti-tag antibody.
> > 
> > I am aware of Brian Kobilka's Anal. Biochem. paper recommending the FLAG
> > tag and anti-FLAG antisera from Eastman Kodak.
> > 
> > All responses appreciated.  Will post tabulated responses for the dining
> > and dancing pleasure of all
> Response:

Why not try the Strep-tag from Biometra. The tag is small and designed
to bind to streptavidin. Good for both detection by Western and afinity

Chris Hoyle

   Department of Biochemistry and Molecular Biology
University of Leeds             phone +44 0113 2333172
Leeds LS2 9JT                   FAX   +44 0113 2333167
Great Britain           e-mail bmbckh at biovax.leeds.ac.uk

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