Epitope Tagging Suggestions Wanted

Dr. Arthur L. Kruckeberg bmbckh at biovax.leeds.ac.uk
Fri Feb 23 12:01:26 EST 1996


In article <312CC8EB.2533 at phy164.physiology.uab.edu>
Wayne Duck <wayne-duck at phy164.physiology.uab.edu> writes:

> A. John Watson wrote:
> > 
> > I am planning to epitope tag one of my favorite receptors and am wondering
> > what systems have people found to be particularly reliable/unreliable with
> > respect to (a) choice of tag sequence and
> > (b) source of anti-tag antibody.
> > 
> > I am aware of Brian Kobilka's Anal. Biochem. paper recommending the FLAG
> > tag and anti-FLAG antisera from Eastman Kodak.
> > 
> > All responses appreciated.  Will post tabulated responses for the dining
> > and dancing pleasure of all
> 
> Response:

Why not try the Strep-tag from Biometra. The tag is small and designed
to bind to streptavidin. Good for both detection by Western and afinity
purification.
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Chris Hoyle

   Department of Biochemistry and Molecular Biology
University of Leeds             phone +44 0113 2333172
Leeds LS2 9JT                   FAX   +44 0113 2333167
Great Britain           e-mail bmbckh at biovax.leeds.ac.uk
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