Annealing temperature of 10-mer oligo

Shaun Tyler styler at HPB.HWC.CA
Sun Feb 25 17:33:54 EST 1996

Sure there are several quick methods for estimating annealing 
temperature.  The easiest is G/C = 4 C and A/T = 2 C.  However, 
regardless of the method you use, an "estimate" is all that you will get. 
 I hate to say it but if you need to be precise there is no alternitive 
to imperical testing.

Shaun Tyler
DNA Core Facility
Laboratory Centre for Disease Control
Health Canada

Ph#:  (613) 941-6441
FAX#: (613) 957-1358

E-mail:  styler at

More information about the Methods mailing list