g/ rpm, pH+temp, V+cm.,conductivity of buffers

WSchick at aol.com WSchick at aol.com
Sun Feb 25 16:57:27 EST 1996


In a message dated 96-02-24 13:07:27 EST, s.faruque at umds.ac.uk (Mr SMNN
Faruque) writes:

>
>Okay, how about people who quote gel conditions in Volts (potential 
>difference) instead of Volt/cm (electric field).
>
>
When doing V/cm, be sure to measure the distance from electrode to electrode,
as the buffer has a resistance too.   
For good measure, one should include the current measurement, as this varies
by thickness of gel, buffer changes, etc.  And some protocols run under
constant current.

Finally on electrophoresis, one should get an inexpensive conductivity meter
to chek their buffers.  Glycine from different sources and even different
lots will show different conductivity, which will change the running time,
etc.  

Walt Schick



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