Monitoring RNA synthesis
nwinegar at erin.utoronto.ca
Mon Feb 26 09:56:01 EST 1996
My apologies if my first post did make it out, however since I received
no replies I thought I'd try again.
I want to measure the synthesis of RNA inside of cells. Specifically, I
would like to be able to quantify and localise the amount of transcript
prepared inside of cells, and I need to perform the localisation by
immunofloresence. Normally I would used tritiated uridine to measure the
amount of synthesis but the resolution of localisation is not high enough
for my needs. I was thinking of using something like DIG-UTP however my
understanding is that this molecule is too large to get into cells. If
at last resort I must I could microinject this molecule, however this
would make my task much more tedious than I hope it to be.
Specifically my question is: Does anyone know of some labelled precursor
that would be taken up by cells? Would DIG-uridine be taken up?
Any help would be greatly appreciated.
Thanks in advance,
University of Toronto - Zoology
nwinegar at credit.erin.utoronto.ca
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