Using glass beads to make E.Coli extracts

MAUR at CNRISC.ISC.MLIB.CNR.IT MAUR at CNRISC.ISC.MLIB.CNR.IT
Mon Feb 26 10:13:44 EST 1996


Dear Netters,
    I am trying to use the Braun MSK Cell Homogenizer as alternative to
sonication to avoid the excess of viscosity of E. Coli extracts due to
intact DNA. So if someone out there could suggest some tips regarding 
paramters to use in cell disruption (such as glass bead diameters, time,
cell concentration, buffer, etc.} the help would be greatly appreciated.

-- Maurizio 



More information about the Methods mailing list