Tommy the Terrorist
u58563 at uic.edu
Mon Feb 26 22:25:43 EST 1996
There's a new protocol out in PNAS (Jan. 23) where you use a restriction
enzyme and the two anchor nucleotides on the oligodT primer to determine
specific products. It avoids low-stringency annealing this way, and
claims to be consistent. They actually cloned some genes with it that do
what they want them to do, too. They reference (by note) another group
who published a similar, but somewhat more sophisticated, procedure in
NAR. The only admitted drawback seems to be that low copy-number
transcripts could be missed.
It *sounds* like fun. But does anyone in the field have any
devastating little comments to make about the procedure?
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