Tet-repressible genes - timing

Med-2.1 Host at cf.ac.uk
Mon Feb 26 13:25:16 EST 1996


Dear Ian,

(1) how long the cells need 
> > to be tetracycline-free before we can be sure they're on,

I can detect expression within 6 hours after tet-removal, but max.
expression is reached after 18-24 hours.

 and (2) 
> > whether we need to be anal-retentive about cleaning out the tet.  Do I 
> > need to wash out the tet medium, or is it likely to be sufficient to 
> > simply remove old medium and replace with new?  
> 

Follow I.Davies advice and wash the cells at least twice in PBS or medium
to get rid of the stuff.
 
Good luck, Eike Floettmann          (Floettmann at CF.AC.Uk)



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