Lambda EMBL 3 primers
carboni at imiucca.csi.unimi.it
Tue Feb 27 03:10:02 EST 1996
does anyone experienced PCR amplification of inserts
in a genomic library in lambda EMBL 3 vector?
If so can someone send me the sequence of PCR primers
designed on the R-arm and L-arm of the vector?
The problem raises from the discovery that for excising
the genomic insert from the recombinant vectors I must use
the RE SalI, wich is sensitive to PEG contamination. In pre-
sence of small traces of PEG, SalI gains the so called 'star
activity', and recognises BamHI and EcoRI sites.
Thats why I obtain, form a single positive colony, a ladder of
I decided to PCR amplify my insert and than clone it in a
Any suggestion would be appreciated.
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