Unspecific hybridization to plasmid-vector

[Tim Barnett]

Is the hybridisation to your insert stronger than that to the vector?  If so you could dilute the digestion products until you only get a signal from the insert.



In article <4gv65s$a8f at rzunews.unizh.ch>, tgerloff at kpt.unizh.ch (Thomas Gerloff) wrote:

> How do I get rid of those unspecific hybridizations and what could be the
> cause?

-- 
Tim Barnett                             fax:   61-02-354833
Department of Pathology                 phone: 61-02-354825
University of Tasmania                  email: t.barnett at path.utas.edu.au
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AUSTRALIA