Request for RELIABLE ssDNA rescue protocol for sequencing.
kulkarnp at coral.indstate.edu
Tue Feb 27 17:27:25 EST 1996
I am doing ssDNA sequencing with Sequenase, and I find that I sometimes do
not get good yields of ssDNA. I use the VCSM13 helper phage from
Stratagene, and have tried growing my clones on Tet plates as they suggested
but yields have not improved. I am looking for a reliable protocol for
ssDNA preps which will give me more consistent results than I am getting
now. If anyone out there has a reliable protocol that they are willing to
share with me, please send it to me at megprasa at thcme.indstate.edu .
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