HELP! GST column with Tris-Saline?

Michael DiDonato michael.didonato at utoronto.ca
Wed Feb 28 10:45:16 EST 1996


I was wondering if it was possible to substitute tris-buffered saline pH
8.0 for PBS pH 7.4 in solubilizing GST fusion proteins and applying them
to the column.  My concern is any affect the tris would have on binding of
the GST fusion to the column.

Any help/advice would be greatly appreciated.

Michael
Department of Biochemistry
University of Toronto
michael.didonato at utoronto.ca



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