Try blotting with 125-I labeled primary antibody only. Alternatively,
use biotin-labeled primary antibody and develop with avidin-enzyme
conjugate (HRP or alk. phos.).
Mark R. Miglarese, Ph.D.
Cancer Research
Pfizer Central Research
Box 372
Eastern Point Road
Groton, CT 06340
Phone: (860) 441-8478
FAX: (860) 441-1290
Email: miglaresemr at pfizer.com