Refolding denatured protein

Alex Chang lpss at unixg.ubc.ca
Thu Feb 29 14:16:30 EST 1996


Thanks for those responded. I have solved the problem by dialysis against 
PBS with decreasing concentrations of Urea. The SFv protein seems to fold 
correctly, because it is active in ELISA assay. 


Alex Chang
Pathology
University of British Columbia
achang at hivnet.ubc.ca



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