How to set up ELISAs

Michael Lassner mikel51 at
Thu Feb 29 01:35:18 EST 1996

I have an affinity purified antibody, and am considering setting up 
an ELISA system to measure antigen levels in developing Brassica 
seeds.  The antibody manual I own looks a little outdated.  Anybody 
have suggestions on the easiest way to set up such a system for 
sure-fire rock-solid results.  Should I use sandwich assays--if so, 
how should I label the antibody.   Are there some microtitre plates 
that bind tens of micrograms of protein that would allow binding the 
plant protein to the plate and then use secondary antibodies?  Any 
suggestions are appreciated.

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