RNA Band problem

Stephanie Smith sls at sanger.ac.uk
Thu Feb 29 05:26:54 EST 1996


Hello,

I've been extracting Total RNA from peripheral blood lymphocytes using a 
general lysis, phenol/chlorophorm extraction method.  To quickly check 
the quality of the RNA, I've been running it quickly on a 1% Agarose 
gel.  I'm seeing the 28s and 18s rRNA bands, BUT I'm also getting a very 
sharp uniform bright band at 20kb+, does anyone have an idea what this 
could be?  I've DNase digested and still get this band.  It's almost 
plasmid like in appearance in the gel.

Thanks for any suggestions, Stephy





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