Problems with Qiagen Maxipreps?
anmab2 at orion.alaska.edu
Wed Feb 28 03:35:09 EST 1996
I haven't done many transfections, but have done a lot of Qiagen maxis.
I know that, for reasons unclear to me, that using home-made buffers,
even if made with the best quality reagents never seem to give DNA of
quite the same quality as that I get using the buffers straight from
Qiagen (based on OD 260/80 ratios), though they work fine for all of the
techniques that I routinely use.
More related to your question: I received a flyer from Qiagen a short
while ago referring to the detrimental effect of endotoxin on
transfection efficiency, which could be part of the problem. Qiagen has
recently started carrying a line of purification buffers that are
certified endotoxin free specifically for purifying plasmid DNAs for
Hope this helps and good luck!
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