Can one gene be used to select for OR against....

Mikhail Alexeyev malexeyev at biost1.thi.tmc.edu
Thu Feb 29 19:52:49 EST 1996


In article <4h5f48$cb5 at hydra.cc.umb.edu>, g1687jkarh at umbsky.cc.umb.edu wrote:

>      Does anyone know of a selectable marker for bacteria (a resistance gene) 
> which confers resistance to one selection agent (antibiotic, etc.) while 
> confering sensitivity to another selection agent? Please respond by EMail. 
>      In other words: I want a gene which if transcribed and translated,
> produces a protein which could be used to select for OR against bacteria
> expressing the gene, depending on which chemical was added to the media. 

That would be a tetracycline-resistance gene. It can be selected against
in the presence of fusaric acid. Aren't you working on IVET by any chance?


M. Alexeyev














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