Methods February 1996 Archives by author

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Starting: Thu Feb 1 00:32:36 EST 1996
Ending: Thu Feb 29 22:21:50 EST 1996
Messages: 1601

Searching forInformation on the Internet "C. S. Prakash", by way of Prakash at acd.tusk.edu C. S. Prakash
Meningococcus "Marianne Leverone ", BIO
Trouble finding DNA concentration on Spec.. "Marianne Leverone ", BIO
TMV "Marianne Leverone ", BIO
DH5a w/o plasmid growing on Amp? "Marianne Leverone ", BIO
Trouble finding DNA concentration on Spec.. Marianne Leverone (BIO)
SYBR Green staining of PAGE/urea gels Juergen Sasse (Path)
Ligation problems on agarose-isolated fragments Rutger Meinsma (procrea)
Subcloning Blues
isolating nuclei from fat tissue Keith Hoek -BIOCHEM
Synercid - (Info) -Help 76342.1257 at compuserve.com
Extracting from agarose (Re: Which kit for cleaning PCR products?) K J C 944
BrdU in immunoflourescence A.S.
PCR problems AJB
murine repetitive DNA Rein Aasland
murine repetitive DNA Rein Aasland
Plasmid Preparation Yasuhito Abe
RNA from mouse skin. David Adelson
Problem with the METHODS newsgroup WAIS index BIOSCI Administrator
METHODS index is functional again BIOSCI Administrator
BIOSCI miniFAQ, ver. 14-DEC-95 BIOSCI Administrator
IMPORTANT - BIOSCI Fundraising Update! BIOSCI Administrator
RNA primed PCR? Adrian.Philbey at SMTPGWY.AGRIC.NSW.GOV.AU
dut- ung- E. coli strain request Adrian.Philbey at SMTPGWY.AGRIC.NSW.GOV.AU
Cells expressing connexin 32? Eva Agurell
Does lipids disturb the binding of DNA & proteins to hydroxylapatite? Eva Agurell
Differential Display Primers Christoph Ahlers
E. coli Promoters Ashok Aiyar
pfu vs taq Ashok Aiyar
dut- ung- E. coli strain request Ashok Aiyar
housekeeping gene rtpcr Bill Alexander
Ligase PCR Bill Alexander
RT-problem in RT-PCR Bill Alexander
bacterial housekeeping mRNA M. Alexeyev
Transformation in CJ236 M. Alexeyev
Deletion dilemma M. Alexeyev
Desalting column Mikhail Alexeyev
Partial RE digestion - please advise! Mikhail Alexeyev
How to covalently link DNA to beads? Mikhail Alexeyev
Plasmid isolation Mikhail Alexeyev
Saturating competent cells with DNA? Mikhail Alexeyev
Can one gene be used to select for OR against.... Mikhail Alexeyev
purification of 140 Kbp from agarose Ghis Allard
source of high quality streptavidin John Altman
tetracyclin inducible system Danny Altshuler
emulgen 911 Marcel Amichot
minimum media Chen Ho An
**ANYONE KNOW SOURCES OF THESE VECTORS?** Eric C. Anderson
Virus dilutions and RT-PCR Anton Andonov
Ancient DNA newsletter? D.L. Andrews
looking for source of cell & tissue extracts Jennifer Anklam
inducible eukaryotic expression Annette
inducible eukaryotic expression Annette
PCR from blood samples Lo Wing Ip Anthony
storing paramortem skin Lo Wing Ip Anthony
miniprep labstation Anuradha
Saccaromyces cerevisiae Tracy Aquilla
Maleic acid buffer: Help me make it Tracy Aquilla
Northern witth oligos Tracy Aquilla
Genetic Evolutionary conservation: Tracy Aquilla
purifying short probes Tracy Aquilla
rat cardiomyocyte cell line, who has one? Tracy Aquilla
Report centrifugal force in g units NOT rpm! (Rant) Curt Ashendel
Overbaked Northern blots Graham Atherton
Rediprime labelling system Cathy Aversa
Hypoxia chamber? Cathy Aversa
(no subject) GANESH BHAT
monomeric avidin BOYDSTON at MVAXGR.HUNTER.CUNY.EDU
Do DNA DE-methylases exist? BSpinoza
Seq. alignment programs? Dan Baggott
Want: Ade2 yeast shuttle vector Dan Baggott
Sequence for AB701? Dan Baggott
Fidelity of polymerase Dan Baggott
How much bis-acrylamide in aa-gel Joseph C. Bagshaw
Acridinium ester labelling Joseph C. Bagshaw
RNA semi-dry electroblotting Joël Baguet
plasmids poly(A) Joël Baguet
Northern blot Joël Baguet
Subcloning Blues Tim Barnett
Need help with non-rad Southerns Tim Barnett
Low stringency Southern trouble Tim Barnett
Qiaex problems Tim Barnett
Unspecific hybridization to plasmid-vector Tim Barnett
nicking DNA plasmids on purpose Tim Barnett
None Chris Barry
Transfection efficiency and cell cylce L. Bastian
Desperately seeking mini "Euro" tissue grinders Michael Bausher
Desperately seeking mini "Euro" tissue grinders Michael Bausher
LacZ reporter stably transfected cells Peggy Bavin
Chemotaxis Cliff Beall
Sources of baculovirus expression systems Cliff Beall
DNA Isolation from whole blood Robin Beech
imaging gels Robin Beech
Lambda DNA prep. Robin Beech
Blatant Selling of Myself! Robin Beech
Re- Purifying DNA for FISH Brett Beitzel
Electrocompetent Cells Brett Beitzel
RNA as blocking agent in PC Brett Beitzel
Re- Rubber Cement on Slides Brett Beitzel
RNA primed PCR? Brett Beitzel
Thermostable RNase Inhibito Brett Beitzel
RNA hydrolysis Brett Beitzel
Chopped RNA Brett Beitzel
Re- Pre-hyb in ISH Brett Beitzel
Blocking transcription? how? Christian Belanger
classical mutagenesis? the best way to do it Christian Belanger
protein-protein interaction and expression libraries. Dr. Jeethan Bendoor
Quick Protein Concentration Assays E.L. Berven
Hall's hyb Beverly Steele Allen, Ph.D.
Peptide sequence immunogenicity B. Ganesh Bhat
tetracyclin inducible system Display Systems Biotech
NEED PCR primer program for MAC David F. Bishop
want comments on phosphorimagers: Molec.Dynamics vs BioRad Bruce Thomas Blakely
Which kit for cleaning PCR products? John Blankenship
housekeeping gene rtpcr Volker Blaschke
Transfection efficiency and cell cylce Volker Blaschke
Transfection efficiency and cell cylce Volker Blaschke
Touchdown PCR protocoll? Volker Blaschke
mung bean nuclease Anna Blom
competent bacteria Anna Blom
TriZol results? Darren Boehning
pfu vs taq Darren Boehning
Anti-Bovine Prolactin Darren Boehning
PSG5vector Torsten Boerchers
Help! Reusing Qiagen-tip Torsten Boerchers
PSG5vector Torsten Boerchers
Hydropathy plot program Torsten Boerchers
rnase free pipet tips Ljubica Bogic
in situ hyb: eosinophilic cell-specific background Ljubica Bogic
Postdoc Salaries? Ljubica Bogic
Electroporation Holger Bohnemeier
Ligation problems on agarose-isolated fragments Anne Bokman
Background in In Situ Hybridizations Reese Bolinger
Problems with Qiagen Maxipreps? Reese Bolinger
problems with RNAs denatured with Glyoxal(in Northerns) Reese Bolinger
Qiagen buffers? Peta C. Bonham-Smith
Nickel agarose for His tag Dorin-Bogdan Borza
PCR templates on Automated DNA sequencers Elizabeth Boulding
PCR templates on Automated DNA sequencers Elizabeth Boulding
NEED PCR primer program for MAC Ian A. Boussy
Oligo 4.0 sofware. Help Ian A. Boussy
quiagen lambda preps Large Hearted Boy
DNA precipitations Chris Boyd
source of lacI repressor wanted Chris Boyd
blunting with S1 nuclease Chris Boyd
DH5a in DMEM Chris Boyd
Protecting PCR runs from blackouts etc. Chris Boyd
Ligase PCR Gerome Breen
Ligase PCR Gerome Breen
WWW Medline Search? Gerome Breen
Sequencing gel question Deb Britt
MacVector-type program for IBM??? Deb Britt
Primer design software Stuart Brown
phage display- deletions Pamela J. Buchli
mRNA from rat brain; seeking best miroscale method Andreas Buhr
New life for old PCR templates? Bill Burnett
Desalting column Dr E. Buxbaum
How to analyze stress proteins Dr E. Buxbaum
RAPDs and PCR Gregory Buzard
mouse Raf genomic clones and sequence wanted Gregory Buzard
pET-vectors Stefan Bäckström
cDNA synthesis from total RNA CHISHOLM at BCRSSU.AGR.CA
Hydropathy plot program Manuel G. CLAROS
Smiles in agarose gels Manuel G. CLAROS
How to covalently link DNA to beads? CPGYIELD
Removing Phenol from Protein Fraction Philip P Calvert
Removing Phenol from Protein Fraction Philip P Calvert
Antibody to Cytosine Deaminase Calydonian
DNA precipitation Hugh D. Campbell
long PCRs: Yes, it works on human DNA ! Eric Campeau
Lambda EMBL 3 primers Alberto Carboni
Nucleolus-specific Antibodies? Carl Costanzi, Ph.D.
Quickest method to visualize plasmid presence in bugs? Philip L. Carl
Qiaex problems Philip L. Carl
Cryomarkers - where can i buy them?? Sara Carlisle
Cryomarkers - where can i buy them?? Sara Carlisle
Partial RE digestion - please advise! Sara Carlisle
Left DNA out at RT...advice? Sara Carlisle
Left DNA out at RT...advice? Sara Carlisle
Cryomarkers - where can i buy them?? Sara Carlisle
Agro. integration into chloroplast DNA Brandt Cassidy
housekeeping gene rtpcr Ed Castro
Transcription: promoter too? Ed Castro
Monoclonal Antibody: commercial purchase Ed Castro
c-jun rat pcr primers Ed Castro
rat cardiomyocyte cell line, who has one? Ed Castro
Maleic acid buffer: Help me make it Rosanne Casu
Suggestions - PCR from Phage Isolates Rosanne Casu
**ANYONE KNOW SOURCES OF THESE VECTORS?** Rosanne Casu
western blot stripping protocol George W Chacko
Refolding denatured protein Alex Chang
Refolding denatured protein Alex Chang
Bioventures Moulliard Charles
Programs for analysing EF hand structure Frank Chen
CNBr activated affinity supports? Yuansha Chen
Using xanthine in cell culture media? Helen Cheng
DIG or Biotin ? LAU Tze Chin
Non-radioactive end-labelling LAU Tze Chin
Southern blot : DIG or BIOTIN?? LAU Tze Chin
Problem with RT-PCR for gene rearrangement LAU Tze Chin
detergent assay Natalie Chinardet
Immunoprecipitation using proteinA agarose Nikolai Chitaev
Maleic acid buffer: Help me make it Nikolai Chitaev
Refolding denatured protein Nikolai Chitaev
zymogram Choiset-Yvan
Deletion dilemma Joe Chou
Subcloning Blues Joe Chou
Wizard maxipreps Lely Chow
Fidelity of polymerase Baumberger Christophe
Subcloning Blues Namjin Chung
making cesium-gluconate solution Dennis Churchill
Meningococcus Dr. Duncan Clark
Inclusion Bodies in E. coli Dr. Duncan Clark
Long PCR Dr. Duncan Clark
Scaling down DNA synthesis on ABI 381 A ? Dr. Duncan Clark
(more info)bacterial housekeeping mRNA Dr. Duncan Clark
Ligase PCR Dr. Duncan Clark
Ligase PCR Dr. Duncan Clark
Using Resources - Apology. Dr. Duncan Clark
Fidelity of polymerase Dr. Duncan Clark
Gene Targeting in Plants (book title...help!) Kathleen Clark
gene targeting in plants (book title) Katie Clark
Deletion Problems using recombinant PCR D L Clarke
Qiagen Oligotex RTPCR protocols wanted D L Clarke
Seeking strange nucleotides (isoCTP and isoGTP) Pierce Chemical Co
Can DTT be substituted by DTE ???? Pierce Chemical Co
Automatic Film Developers? Pierce Chemical Co
Western blot mystery Pierce Chemical Co
Sulfate uptake inhibitor: Help Michael Coady
magnetic bead losses during manipulation Wayne Coco
Looking for T7 gene6 exonuclease Wayne Coco
longevity of TAQ!! Problems with Pharmacia Wayne Coco
Fluorometer: to buy or not? Christopher T. Cole
TESTXXX Lindsay Collinson
TESTXXX Lindsay Collinson
TESTXXX Lindsay Collinson
TESTXXX Lindsay Collinson
TESTXXX Lindsay Collinson
(no subject) Lindsay Collinson
HELP!! pcDNAI, pcDNA1/AMP : expression cloning Lindsay Collinson
HELP!! pcDNAI, pcDNA1/AMP : expression cloning Lindsay Collinson
Why NH4 NO3 in Silver Staining Pierre Commercon
removing Rubber cement on slides Pierre Commercon
Antibody against Foetal Hemoglobin Pierre Commercon
Fluorescence spectrum of DNA Pierre Commercon
RT PCR from a drop of blood Pierre Commercon
Fluorometer: to buy or not? Computer
Non-radioactive end-labelling Computer
Fluorometer: to buy or not? Computer
removing Rubber cement on slides Computer
Magnesium effects on PCR RAY-Francois Comte
primer extension anyone ? RAY-Francois Comte
Sequencing gel question RAY-Francois Comte
where can I get PAP RAY-Francois Comte
Long PCR ConstantinPolychronakosmc97 at musica.McGill.CA
(no subject) Richard Converse
Sources of Anti-LexA Seth Corey
Do DNA DE-methylases exist? Joao Vasconcelos Costa
pfu vs taq Joao Vasconcelos Costa
PCR primer software Joao Vasconcelos Costa
MICRODISSECTION OF CHROMOSOMES Andrea Crampton
need info on hygromycin Mark Crowe
GAANN Assistantships in Biology Theodore C. Crusberg
protein-protein interaction and expression libraries. Joaquim Culi
Chemotaxis Fiona Culley
Wanted: Porcine ovarian cDNA library Jim Cummins
chart recorder ink Angela D'Aprile
Any better PVDF membrane for small peptides? Where? Dr. D.H.Wu
technician position DAB at CNSVAX.ALBANY.EDU
Spec interfaced with Mac DAB at CNSVAX.ALBANY.EDU
Mycoplasma treatment Eric DELABESSE
protocols Peruzan Dadbeh
DNA from spores Peruzan Dadbeh
"Stratacooler" sources? Richard Daines
Low stringency Southern trouble Richard Daines
Problems with Qiagen Maxipreps? Richard Daines
Subcloning large PCR products Daniel
Boiling DH5a Dans
Biotinylating peptides David L. Haviland, Ph.D.
Coating-pH ? David L. Haviland, Ph.D.
primer extension anyone ? David L. Haviland, Ph.D.
Coating-pH ? David L. Haviland, Ph.D.
Coating-pH ? David L. Haviland, Ph.D.
purifying short probes David L. Haviland, Ph.D.
DH5a w/o plasmid growing on Amp? David L. Haviland, Ph.D.
Rep (3) : multiple m-RNA measurement Mr SHIRE David
Rep (4) : multiple m-RNA measurement Mr SHIRE David
RNA isolation from plants George Davis
blocking in northern blots Elliott Dawson
Help Elliott Dawson
Microtitre plates: edge effects? Elliott Dawson
Biotin-PEG-NHS Elliott Dawson
Glycerol inhibition of PCR? Elliott Dawson
bacterial housekeeping mRNA Elliott Dawson
DTT Stability Elliott Dawson
bacterial housekeeping mRNA Elliott Dawson
(more info)bacterial housekeeping mRNA Elliott Dawson
help with "PNA", a DNA with peptide backbone Elliott Dawson
competent bacteria Simon Dawson
Job Post: 3 Positions (IRCM) Montreal, Canada Graham Dellaire
Which is the best Method for Purifying DNA for FISH analysis?? Graham Dellaire
Puromycin Concentration for Selection in Mouse Fibroblasts? Graham Dellaire
Job Listing (Lady Davis Institute (Montreal... McGIll University)) Graham Dellaire
Jobs in MOntreal (today's post is for a Ph.D. position 5 Year MRC) Graham Dellaire
Job Listings for Montreal... Ph.D. at the Royal Victoria Graham Dellaire
PNA's Graham Dellaire
HAs anyone heard of or used MAC-PRobe for FISH analysis??? Graham Dellaire
Differential display kit: who is selling it? Deneke at UTHSCSA.edu
Genehunter Deneke at arwen.UTHSCSA.edu
glyoxalation of RNA R. Rex Denton
HELP! GST column with Tris-Saline? Michael DiDonato
Ligation problems on agarose-isolated fragments Catherine I. DiOrio
Ligation problems on agarose-isolated fragments Catherine I. DiOrio
EGF receptor ab Catherine I. DiOrio
Bovine housekeeping gene Linda DiPersio
Immunoprecipitation using proteinA agarose Eddie Diehl
DTT Stability Eddie Diehl
Double Labeling Problem Stephanie Dillon
Use a genomic clone to find the cDNA John Dixon
Partial RE digestion - please advise! John Dixon
Cre recombinase John Dixon
Partial RE digestion - please advise! John Dixon
Custum oligo on magnetic beads John Dixon
Easy method for size fractionation of genomic digests? John Dixon
Saturating competent cells with DNA? John Dixon
Help with Native PAGE Alvin Dobbin
anti CAT antibody??? Geoffrey Doerre
anti CAT antibody??? Geoffrey Doerre
LacZ stably transfected cell line Jeanmarie Donovan
housekeeping gene rtpcr Peter Doris
two-hybrid, discrepancies Iris Dotan
THYMIDINE FREE MEDIA Tom Dougherty
competent bacteria Tom Dougherty
E.coli strain availability? Tom Dougherty
Differential Display Dr N.J. Doyle
Ligation problems on agarose-isolated fragments Paul S. Drayna
Rapid Ligation Paul S. Drayna
what's an easy way to look for cell death? Paul S. Drayna
GFP Tomas Drgon
GFP truncation - summary Tomas Drgon
Electroporation of 3T3 fibroblasts Antonio Duarte
Trouble finding DNA concentration on Spec.. Wayne Duck
Monoclonal Antibody: commercial purchase Wayne Duck
Epitope Tagging Suggestions Wanted Wayne Duck
Epitope Tagging Suggestions Wanted Wayne Duck
PSG5vector Johanne Duhaime
Refolding denatured protein Tim Dunn
NIH units of opacity Ken Eastwell
Coating-pH ? Mad Dan Eccles
DTT Stability Mad Dan Eccles
G418 Mad Dan Eccles
subscribe Molecular Genetics in Ecology
Quantitation of SDS-PAGE George F Edick
Fucus DNA extraction Gareth Edwards
extracting RNA from bone SG Edwards
PCR contaminant with abs peak 299 SG Edwards
(none) Margaret Egli
Using Biotin in ELISA's Karl Eisele
Apomorphine ramon dos Santos El-Bacha'
Promega Wiz Preps increase yield Elke
Ligation problems on agarose-isolated fragments John Elliott
Smear in gel lanes (mini preps) John Elliott
dut- ung- E. coli strain request John Elliott
DH5a w/o plasmid growing on Amp? John Elliott
SENSE vs. ANTI-SENSE DNA CALL FOR VOTES the End
SENSE vs. ANTI-SENSE DNA CALL FOR VOTES the End
Smear in gel lanes (mini preps) the End
Transformation in CJ236 the End
Genescreen+ vs. Hybond N+ ? the End
SENSE vs. ANTI-SENSE DNA CALL FOR VOTES the End
peptide antigen success rate Steven Enkemann
need fusion protein for protein kinase substrate Steven Enkemann
Summary: Protein dimerisation in SDS gels Lutz Essers
Background in In Situ Hybridizations AMY A. FLUET
100bp molecular weight marker Glibert Fabrice
housekeeping gene rtpcr Patrick HJ Falckh
primer extension anyone ? Patrick HJ Falckh
(no subject) Patrick HJ Falckh
cDNA Library Screening Texts Patrick HJ Falckh
Ethidium bromide and clothes Patrick HJ Falckh
capillary PCR machine Patrick HJ Falckh
Prerunning gels Mr SMNN Faruque
dna diffusion in agarose Mr SMNN Faruque
Gut Protein extracts (+DNA) Mr SMNN Faruque
Gut Protein extracts (+DNA) Mr SMNN Faruque
Gut Protein extracts (+DNA) Mr SMNN Faruque
Smiles in agarose gels Mr SMNN Faruque
g/ rpm, pH+temp, V+cm. Mr SMNN Faruque
Ligation problems on agarose-isolated fragments Mr SMNN Faruque
immunoprecipitations in thymocytes.... Stacy Ferguson
Genomic S. blot problems Karen Ferri
DNA sequencing problem Heather Finnerty
NTA Ni chelator for coupling? H. David Fischer
Subcloning Blues Karl Fischer
Transfection efficiency and cell cycle Karl Fischer
Nru I digestion problems Karl Fischer
Help Tim Flanigan
dut- ung- E. coli strain request George R. Flentke
Looking for T7 gene6 exonuclease Parke K. Flick
Warning! More Membrane SWAPs Parke K. Flick
Chromosomal location of muscarinic receptor genes David Fogarty
home-made mouse cot1 DNA Jiri Forejt
DAPI/Calcofluor SL Forsburg
non radioactiv cAMP measurement Michael Forte
cDNA libraries from MOLT-4 and A549 Michael Forte
How to analyze stress proteins Carola Fortelius
How to analyze stress proteins Carola Fortelius
unable to amplify a polymorphism in the APOC3 gene Pierre Fouret
DNA Synthesizer info wanted John E. Fox
Refolding denatured protein Lee Fradkin
E.coli strain availability? Michael R. Frank
Lambda DNA prep. Amie Franklin
mung bean nuclease Greg Fransen
Staining DNA in Agarose with Methylene Blue S. M. Fullerton
Antibody against serpin-enzyme complex (SEC) receptor anywhere? G.P.
(none) STEVEN GERHARDT
(none) STEVEN GERHARDT
terminology STEVEN GERHARDT
nuclease S1 Pascale GOUPIL
DdeI won't cut PCR product GRIFFRK at wvnvm.wvnet.edu
Report centrifugal force in g units NOT rpm! (Rant) Warren Gallin
electroporation of proteins ? Xavier Gansel
expression vectors Arturo Garcia-Horsman
expression vectors Arturo Garcia-Horsman
Protein Nucleic Acids R. Dana - Green Gene
Boiling miniprep problems with DH5-alpha Saverio Brogna Genetics
problems with RNAs denatured with Glyoxal(in Northerns) Saverio Brogna Genetics
Blatant Selling of Myself! Szatmari George
Unspecific hybridization to plasmid-vector Thomas Gerloff
Unspecific hybridization to plasmid-vector Thomas Gerloff
Seq. alignment programs? Don Gilbert
removal of rRNA Michael Gillings
RAPDs and PCR Michael Gillings
REPLICA PLATING ES CELLS? Bert Gold
sequencing gels won't stick!! Steven Goldberg
Green-Fluorescent Protein Purification Short Course Daniel Gonzalez
DTT Stability Frank R. Gorga
Placental cells isolation Marat Gorivodsky
bacterial housekeeping mRNA Michael C. Gorry
bacterial housekeeping genes Michael C. Gorry
Do NTP's precipitate with ethanol? How efficiently? Anton Scott Goustin
(no subject) J. Arturo Gracía-Horsman
(no subject) J. Arturo Gracía-Horsman
Diatom/Celite David Grant
Unspecific hybridization to plasmid-vector David Grant
housekeeping gene rtpcr Sam Gray
housekeeping gene rtpcr Sam Gray
Anti Tight Junction mAb Anthony Griffiths
ANTISENSE DISCUSSION Duke Groebe
DNA from nitrocellulose for PCR Duke Groebe
Square Phage plating plates. Mike Gruidl
Boiling miniprep problems with DH5-alpha Wenjin Guo
JOB - CA - La Jolla - Lab. Devel. Biochemist Douglas Gurevitch
JOB - CA - La Jolla - Software. Systems Engineer Douglas Gurevitch
amplified inserts Ferland Louis H.
antisense delivery by liposomes Ferland Louis H.
genetic evolutionary conservation Ferland Louis H.
competent bacteria Ferland Louis H.
Halls hyb? Ferland Louis H.
Northern witth oligos Ferland Louis H.
Annealing temperature of 10-mer oligo Ferland Louis H.
Protein gel Ferland Louis H.
Platinum wire for electrophoresis in France H.M
lif-faktor 4 es-cells H.M
Plasmid Drawing Programme HARVEY at BBSRC.AC.UK HARVEY
Lambda YES vector E. Hack
peptide antigen success rate Allison Haggarty
DNA from whole blood Sabine Hanelt
CD and fluoro spectroscopy of tertiary structure Michael Hanlon
HELP please: Inverse PCR with genomic fragments! Burkhard Hassel
polishing PCR fragments with T4 Pol ? Burkhard Hassel
Rapid Ligation Burkhard Hassel
Rapid Ligation Burkhard Hassel
Alternative Methods for Preparing Lifts Shoji Hatano
Maleic acid buffer: Help me make it Shoji Hatano
One-Hybrid Joerg Hauf
Puromycin Concentration for Selection in Mouse Fibroblasts? Christoph Haupenthal
Puromycin Concentration for Selection in Mouse Fibroblasts? Christoph Haupenthal
Puromycin Concentration for Selection in Mouse Fibroblasts? Christoph Haupenthal
Searching for mouse cell lines with high and low MHC I expression Christoph Haupenthal
PCR trouble!!! MV. Hayes
PCR trouble!!! MV. Hayes
pfu vs taq MV. Hayes
Antibody dimer/monomer MV. Hayes
Cutting PCR-created restriction sites MV. Hayes
Using Biotin in ELISA's JG. Head
DNA Isolation Kit Amos Heckendorf
Custum oligo on magnetic beads Helmut
SENSE vs. ANTI-SENSE DNA CALL FOR VOTES Paul N Hengen
SENSE vs. ANTI-SENSE DNA CALL FOR VOTES Paul N Hengen
antisense delivery by liposomes Paul N Hengen
Tracking dyes in agarose gel electorphoresis Paul N Hengen
Any other way of examining biosci archive..? Paul N Hengen
ANTISENSE DISCUSSION Paul N Hengen
Non-radioactive end-labelling Paul N Hengen
competent bacteria Paul N Hengen
100 bp molecular weight marker Paul N Hengen
"easy open" tubes Paul N Hengen
complete removing of primer Paul N Hengen
Ethidium Bromide Paul N Hengen
Hydropathy plot program Paul N Hengen
SENSE vs. ANTI-SENSE DNA VOTING RESULTS Paul N Hengen
DH5a w/o plasmid growing on Amp? Paul N Hengen
Saturating competent cells with DNA? Paul N Hengen
Fidelity of polymerase Paul N Hengen
Alkaline Phosphatase substrates Paul N Hengen
dut- ung- E. coli strain request username at BBSRC.AC.UK Your Name Here
"Stratacooler" sources? Russ Herwig
Help: Degree of Differentiation Rachmat Hidajat
Coating-pH ? Steve Hillerman
expression systems Miss. V.M. Hodges
Removing Phenol from Protein Fraction Flip Hoedemaeker
Help with He sparging of HPLC solvents Robert D. Hoffman
DNA extraction/PCR from dried samples Susan Jane Hogarth
DTT Stability Susan Jane Hogarth
Ethidium bromide and clothes Susan Jane Hogarth
Smiles in agarose gels Susan Jane Hogarth
Q:Purification of total nucleic acid and protein Dave Hollingworth
Purifiction of total nucleic acid and protein. How?? Dave Hollingworth
Do NTP's precipitate with ethanol? How efficiently? Dave Hollingworth
NTP precipitation. Wrong end of the stick!! Dave Hollingworth
Custum oligo on magnetic beads Curtis A. Holt
Primers storage.. Curtis A. Holt
what's an easy way to look for cell death? Louis Hom
Ethidium bromide and clothes Hopkins, Charlotte
Designing primers Hopkins, Charlotte
Ganciclovir and HeLa Cells - help! Henry Hoppe
Ligation problems on agarose-isolated fragments Peter Horn
SENSE vs. ANTI-SENSE DNA CALL FOR VOTES Robert Horton
"Stratacooler" sources? Robert Horton
Long PCR Robert Horton
PCR from blood samples Robert Horton
Seeking strange nucleotides (isoCTP and isoGTP) Robert Horton
RNA primed PCR? Robert Horton
capillary PCR machine Robert Horton
Freshman chemistry Robert Horton
RNA from mouse skin. Robert Horton
custom service: transgenic mices Robert Horton
link two DNAs-a better idea needed Robert Horton
Nickel agarose for His tag Eugenio L. de Hostos
GFP antiserum Eugenio L. de Hostos
quantitative RTPCR from direct cell lysate? Fang-Yao Hou
Specific MAPK-K inhibitor Alan K. Howe
SENSE vs. ANTI-SENSE DNA CALL FOR VOTES Ken Howe
link two DNAs-a better idea needed Wei Hu
determination of binding constant of protein-ligand Baohua Huang
Refolding denatured protein IJ Huang
peptide antigen success rate IJ Huang
Announcement: Details of second prediction experiment, CASP2 Tim Hubbard
How to modify a DNA construct? Martin Hughes
Any better PVDF membrane for small peptides? Where? Jim Hutchins
antisense delivery by liposomes Iddo
juks Rainer Ilisson
"Stratacooler" sources? Michael Irwin
Superbroth for EVERYONE! (NOT) David Ish-Horowicz
Superbroth for EVERYONE! (NOT) David Ish-Horowicz
Superbroth for EVERYONE! (was NOT, but now IS) David Ish-Horowicz
Background in In Situ Hybridizations David Ish-Horowicz
Which kit is recommended for differential display ? Toshiharu Ishizuka
test Antonio Izzo
(none) MUCHA JAN
protocal for staining isozymes JMonaghan
Help with He sparging of HPLC solvents Jafinlay
Alternative Methods for Preparing Lifts K.D. James
Qiaex problems K.D. James
unsubscribe James_Hayden at bio-rad.com
Wheat Callus Suspension... Vasantharajan Janakirahan
DTT Stability Friedemann Janus
double-annealing problem Jean.Mariaux at zool.unine.ch
Deuterium oxide in density gradient? Csaba Jeney
centrif. force: g or rpm? and pH and temp. Csaba Jeney
REPLICA PLATING ES CELLS? Jfloring at aol.com
None Changcun Jia
How to modify a DNA construct? Changcun Jia
CPP-32 antibody William Jia
bacterial housekeeping genes Jim
NEED PCR primer program for MAC Jim Sutcliffe, Ph.D.
Postdoc Salaries? John W. Adams, Ph.D.
Postdoc Salaries? John W. Adams, Ph.D.
Antibody for Beta-galactosidase Doug Johnson
RAPDs and PCR Doug Johnson
Maleic acid buffer: Help me make it Scott C. Johnson
superbroth protocol finally finished Scott C. Johnson
isopropanol to precipitate DNA: how much? (Dave Johnston)
Scanning densitometry problem (Dave Johnston)
Maleic acid buffer: Help me make it Jon
What format is the Superbroth file? Chris Jones
In situ hyb with Digoxigenin Liz Jones
In situ hyb with Digoxigenin Liz Jones
Ligase PCR Zophonias O. Jonsson
Blatant Selling of Myself! Zophonias O. Jonsson
Ligase PCR Zophonias O. Jonsson
Using Resources - Apology. Zophonias O. Jonsson
link two DNAs-a better idea needed Zophonias O. Jonsson
Sequencing gel question Rob Jordan
Advice on genomic DNA preps wanted Rob Jordan
Protein w/ no Trp quantitation: help!!! Luca Jovine
Saccaromyces cerevisiae Jose Ribamar dos Santos Ferreira Junior
Request for RELIABLE ssDNA rescue protocol for sequencing. Isabelle Jupin
isopropanol to precipitate DNA: how much? K
Bioventures Gary K.
isopropanol to precipitate DNA: how much? Gary K.
How to modify a DNA construct? Gary K.
Rapid Ligation Gary K.
Address of Novagene ?? Gary K.
Rapid Ligation Gary K.
Source for Cre Recombinase ? Gary K.
dna diffusion in agarose Gary K.
Nru I digestion problems Gary K.
Fill-in reaction Gary K.
Quickest method to visualize plasmid presence in bugs? DANIEL Y KIM
protein-protein interaction and expression libraries. DANIEL Y KIM
Square Phage plating plates. DANIEL Y KIM
Platinum wire for electrophoresis in France DANIEL Y KIM
Ethidium bromide and clothes DANIEL Y KIM
Rediprime labelling system Mary Kafka
[Q]Immunostaining of rat heart tissue Stefan Kahlert
Expression vector Makoto Kanzaki
DH5a in DMEM David Karaolis
Polymorphism David Karaolis
DH5a in DMEM David Karaolis
Introduction of stop codons J. Kaufmann
advice on genomic DNA prep Keith
Endocytosis in cell culture Nigel Kenward
Rapid Ligation Nigel Kenward
Rapid Ligation Nigel Kenward
Expression vector containing SV40 large T antigen? Nigel Kenward
Qiaex problems Nigel Kenward
nonradioactive ish Gabriele Kerber
RABBIT anti-mouse IgM Gabriele Kerber
RABBIT anti-mouse IgM Gabriele Kerber
RABBIT anti-mouse IgM Gabriele Kerber
non radioactiv in situ hybridization Gabriele Kerber
Cryomarkers - where can i buy them?? Rich Kernan
Maleic acid buffer: Help me make it Rich Kernan
HELP:Saving IEF gel KevO
Hydropathy plot program Kiley
what's an easy way to look for cell death? Desok Kim
SENSE vs. ANTI-SENSE DNA CALL FOR VOTES Dima Klenchin
electroporation of mammalian cells Dima Klenchin
Apyrase - concentrations, storage Dima Klenchin
source of high quality streptavidin Dima Klenchin
NATIVE GEL ELECTROPHORESYS ANSWER Dima Klenchin
Using Resources - Apology. Dima Klenchin
Protein w/ no Trp quantitation: help!!! Dima Klenchin
Using Resources - Apology. Dima Klenchin
Transfection efficiency and cell cylce Dima Klenchin
superbroth protocol finally finished Dima Klenchin
Biogel Dima Klenchin
What format is the Superbroth file? Dima Klenchin
DNA Synthesizer info wanted Stefan J Kley
Designing primers Dave Knorr
dna diffusion in agarose Riekeltje Koedood
Ligation problems on agarose-isolated fragments Riekeltje Koedood
Ethidium Bromide Roland Koelliker
DNA storage Roland Koelliker
Sources for GFP's Stephen Kolaczkowski
purchasing power supply for sequencing Alexander Kraev
LI-COR automatic sequencer? Alexander Kraev
Can seq gel sit O/N before drying? Alexander Kraev
LI-COR automatic sequencer? Alexander Kraev
LI-COR automatic sequencer? Alexander Kraev
Maleic acid buffer: Help me make it Alexander Kraev
Sequencing gel question Alexander Kraev
Sequence of Lambda DASH II polylinker Alexander Kraev
nicking DNA plasmids on purpose Alexander Kraev
Epitope Tagging Suggestions Wanted Cornelius Krasel
RT-problem in RT-PCR Thomas Kreuzer
staining of phosphoproteins Jens Krieger
Quantitation of SDS-PAGE Rajesh Krishnamurthy
Transcription factors and transfections Karen Kroeger
Transcription factors and transfections Karen Kroeger
Magnesium effects on PCR Dubear Kroening
Epitope Tagging Suggestions Wanted Arle Kruckeberg
lipid phase transition temperature Arle Kruckeberg
Ligation problems on agarose-isolated fragments Dr. Arthur L. Kruckeberg
Mung Bean Nuclease Dr. Arthur L. Kruckeberg
Subcloning Blues Dr. Arthur L. Kruckeberg
Epitope Tagging Suggestions Wanted Dr. Arthur L. Kruckeberg
Qiaex problems Dr. Arthur L. Kruckeberg
cDNA & long transcripts Warren D. Kruger
competent bacteria Warren D. Kruger
PCR Frustrations Warren D. Kruger
Polymorphism Warren D. Kruger
Request for RELIABLE ssDNA rescue protocol for sequencing. Prasad Kulkarni
primer extension anyone ? A. Kumar
Yeast transformation by electroporation Li-yun Kung
Long PCR Chris Kyle
Homology Search Software? martin LEACH
Protein Crystallization Supplies martin LEACH
Promoter elements Database???? martin LEACH
DNA from whole blood martin LEACH
promoter sequences martin LEACH
promoter sequences martin LEACH
Microtitre plates: edge effects? martin LEACH
Gelshift: bands w/o extract? martin LEACH
Amersham WWW Site Open!!! martin LEACH
Anyone with a WWW page...please read martin LEACH
Blatant Selling of Myself! martin LEACH
Blatant Selling of Myself! martin LEACH
Using Resources - Apology. martin LEACH
DNA Synthesizer info wanted martin LEACH
luciferase vectors martin LEACH
MacVector-type program for IBM??? martin LEACH
Mitochondrial DNA extraction? martin LEACH
complete removing of primer martin LEACH
Cutting PCR-created restriction sites martin LEACH
Low stringency Southern trouble martin LEACH
Blatant Selling of Myself! martin LEACH
Blatant Selling of Myself! martin LEACH
Blatant Selling of Myself! martin LEACH
Freshman chemistry martin LEACH
Transfection efficiency and cell cylce martin LEACH
multiple m-RNA measurement martin LEACH
nuclease S1 martin LEACH
selling of oneself martin LEACH
selling of oneself martin LEACH
Looking for Sequencing Service Company martin LEACH
Genomic S. blot problems martin LEACH
Searching forInformation on the Internet martin LEACH
How to covalently link DNA to beads? martin LEACH
HAs anyone heard of or used MAC-PRobe for FISH analysis??? martin LEACH
How to covalently link DNA to beads? martin LEACH
Advice on genomic DNA preps wanted martin LEACH
purifying short probes martin LEACH
**ANYONE KNOW SOURCES OF THESE VECTORS?** martin LEACH
Chromosomal localization martin LEACH
Problems with Qiagen Maxipreps? martin LEACH
Left DNA out at RT...advice? martin LEACH
Primers storage.. martin LEACH
Address of Pharmigen San Diego, CA Chih-Sheng LI
rnase free pipet tips Morrison Lab
Nitrocellulose Phage Lifts-- Reprobing? The Wagner Lab
digital cameras The Wagner Lab
dut- ung- E. coli strain request The Wagner Lab
HELP please: Inverse PCR with genomic fragments! John Ladasky
Wanted : Transfection References, Advice John Ladasky
Ancient DNA newsletter? John Ladasky
How much bis-acrylamide in aa-gel Mark Lamkin
5' biotinylation of RNA Marc Lamphier
How to covalently link DNA to beads? Marc Lamphier
Differential Display Marc Lamphier
Magnesium effects on PCR Stephen Marcus Lane
Fluorometer: to buy or not? Stephen Marcus Lane
meeting announcement: Gene Expression Interrelationships James Larkin
Meeting announcement: DNA Forensics James Larkin
Promoter elements Database???? Stephen R. Lasky
SENSE vs. ANTI-SENSE DNA CALL FOR VOTES Stephen R. Lasky
Coating-pH ? Stephen R. Lasky
antisense delivery by liposomes Stephen R. Lasky
ANTISENSE DISCUSSION Stephen R. Lasky
Fill-in reaction Stephen R. Lasky
Exponentially growing U937 ?? Stephen R. Lasky
influence of ethanol Stephen R. Lasky
Does Anybody Have An Answer Stephen R. Lasky
tetracyclin inducible system Stephen R. Lasky
Genomic S. blot problems Stephen R. Lasky
IGF Type 1 receptor cDNA Stephen R. Lasky
In-situ Protein Kinase C assay Bernard Lassegue
PCR Frustrations Dan Lasser
probs digesting plant genomic Michael Lassner
Question: lambda plaques not transparent, bacterial lawn not opaque Michael Lassner
How to set up ELISAs Michael Lassner
complete removing of primer Kyung-Kwon Lee
Ligation problems on agarose-isolated fragments Philippe Lefebvre
PSG5vector Philippe Lefebvre
PSG5vector Philippe Lefebvre
Good inhibitors to the CIP Philippe Lefebvre
determination of binding constant of protein-ligand Philippe Lefebvre
Differential display kit: who is selling it? Philippe Lefebvre
Address of Novagene ? Philippe Lefebvre
epitope tagging Alfredo Lopez De Leon
Rapid Ligation Victor Levenson
Partial RE digestion - please advise! Victor Levenson
Transfection efficiency and cell cylce Victor Levenson
DNA sequencing problem Dale Levitzke
"easy open" tubes David N. Levy
Cymbus Biosciences, Ltd. address David N. Levy
**ANYONE KNOW SOURCES OF THESE VECTORS?** David N. Levy
Superbroth for EVERYONE! sprbrth2.zip - sprbrth2.zip (0/1) David N. Levy
Why oligos accumulated in nucleus Haitao Li
mRNA from rat brain; seeking best miroscale method Jonas Lindeberg
Where to get FIAU (for knockouts) ? Jonas Lindeberg
Where to get FIAU (server down - pls repeat answers) Jonas Lindeberg
Anti mouse FAS antibody Nigel Lindsey
Lambda EMBL 3 primers Xiaoying Lin~{AVO~S0~}
Cloning a fragment from a gel Jan Liphardt
Thioredoxin fusion system Stanley Liu
Ligation problems on agarose-isolated fragments Stanley Liu
Boeringer-Manneheim cycle sequencing Stanley Liu
what's an easy way to look for cell death? Richard A. Lockshin
DNA extraction of woody plant Muhammad A. Lodhi
DIG labelling Benke/Belmar Login
Alkaline Phosphatase substrates Ben Long
Southern blot : DIG or BIOTIN?? LouPass
Dye and terminator chemistry Bunny Lover
Help! Reusing Qiagen-tip Morten Lukacs
pH Meter Adrian Lund
help with "PNA", a DNA with peptide backbone Xiao-zhong Jams Luo
Epitope Tagging Suggestions Wanted Jennifer Lyon
PCR problems Ted M.
Superbroth for EVERYONE! (was NOT, but now IS) Ted M.
DH5a w/o plasmid growing on Amp? Ted M.
Problem with dephosphorylation M.Albrecht
cDNA-Library of granulocytes M.K.
cDNA-Library of granulocytes M.K.
Using glass beads to make E.Coli extracts MAUR at CNRISC.ISC.MLIB.CNR.IT
Cell lysates for bFGF-How? N.Ambalavanan MD
Marker for cell death? N.Ambalavanan MD
Desalting column N.Ambalavanan MD
Porcine EGF assay? N.Ambalavanan MD
Looking for information on OKT3 antibody Paul C. Grimm MD
capfinder NEAL MUSTO
RNA hydrolysis Clinton MacDonald
Molbio TOP TIPS John MacFarlane
complement activation David Macdonald
DNA packaging in M13 Lee V. Madrid
Transgenic rat cooperation Josef Peter Magyar
mRNA isolation in E. coli Rafael Maldonado
DIG labelling Rafael Maldonado
Blocking transcription? how? Rafael Maldonado
classical mutagenesis? the best way to do it Rafael Maldonado
arrested nascent chains (fwd) Rafael Maldonado
complete removing of primer Rafael Maldonado
complete removing of primer (CORRECTION) Rafael Maldonado
used equipment Dean Malencik
Genehunter Sanjay Mallya
Biology/Chemistry Journals Free to Univ. Library Stephen Mamber
isolating nuclei from fat tissue Michael Man
Tracking dyes in agarose gel electorphoresis Ned Mantei
DTT Stability Ned Mantei
Partial RE digestion - please advise! Ned Mantei
nicking DNA plasmids on purpose Ned Mantei
Cutting PCR-created restriction sites Marc J. Mass, Ph.D.
Antibody dimer/monomer Jose F Gutierrez Marcos
Buffer PN from Quiagen? Jose F Gutierrez Marcos
Buffer PN Jose F Gutierrez Marcos
Problems with Qiagen Maxipreps? Jeremy Marcus
Light Sensitivity of Tris Jeremy Marcus
Abbreviated miniprep protocol... Incredible! Jeremy Marcus
VCAM-1 promoter Marion C. Cohen, Ph.D.
DAPI/Calcofluor Nancy A. Markley
test Paula Marquardt
Extracting from agarose (Re: Which kit for cleaning PCR products?) David Martin
determination of binding constant of protein-ligand David Martin
Do NTP's precipitate with ethanol? How efficiently? David Martin
Using Biotin in ELISA's David Martin
Address of Novagene ?? Martin_Messerle
Source for Cre Recombinase ? Martin_Messerle
Address of Novagene ? Martin_Messerle
Partial digest - Addition of Et.Br. Martin_Messerle
Light Sensitivity of Tris J. Pat Martinez
bovine pitutary extract Mary Kay Matthys
Classical mutagenesis Helen McBride
Wanted: His Antibodies Mark McCormick
HELP! Problem with Pharmacia's Genequant Spec Julie McGrew
Transient transfection of U937 and THP-1 Willie McPheat
Transient transfections of U937 and THP-1 Willie McPheat
Tet-repressible genes - timing Med-2.1
Superbroth for EVERYONE! (was NOT, but now IS) Ian J. Mehr
Biogel P. Mertens
Refolding denatured protein P. Mertens
Meningococcus Alex Merz
Blocking Phosphotyrosine Blots Alex Merz
Biotin-PEG-NHS Mary Meza
Rapid Ligation Ted Michelini
100bp molecular weight marker Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
Bioline David Micklem
Which kit for cleaning PCR products? Microplate at eworld.com
Chemotaxis Microplate at eworld.com
Custum oligo on magnetic beads Microplate at eworld.com
competent bacteria Mark R. Miglarese
Partial RE digestion - please advise! Mark R. Miglarese
Suggestions - PCR from Phage Isolates Mark R. Miglarese
luciferase vectors Mark R. Miglarese
Ligation problems on agarose-isolated fragments Mark R. Miglarese
(no subject) Mark R. Miglarese
Problems with Qiagen Maxipreps? Mark R. Miglarese
Nuclear run-on assays Mark R. Miglarese
Help? Immunoprecipitation Mark R. Miglarese
Help? Immunoprecipitation Mark R. Miglarese
RNA isolation Mark R. Miglarese
freeze-squeeze Mark R. Miglarese
Trouble finding DNA concentration on Spec.. Charles A Miller
PCR, Tm, and ligations..? Charles A Miller
What format is the Superbroth file? Charles A Miller
What would the lacZa fragment do in a protein fusion? Charles A Miller
Can DTT be substituted by DTE ???? Giampaolo Minetti
Ab against Angiostatin Thomas Mohr
Which kit for cleaning PCR products? Moosemeister
digital cameras David D Morgan
DNA analysis software Morlute
Stratacooler souces? Mark D Morris
Question about serial analysis of gene expression (SAGE) Stephen Morris
hybridoma search Heidi Moss
primer extension Heidi Moss
luciferase vectors Heidi Moss
How much bis-acrylamide in aa-gel Dirk Mueller
Taq error "spectrum" Bernard Murray
blocking in northern blots Bernard Murray
QUANTITATIVE PCR Bernard Murray
Any other way of examining biosci archive..? Bernard Murray
Non-radioactive end-labelling Bernard Murray
PSG5vector Bernard Murray
Maleic acid buffer: Help me make it Bernard Murray
PSG5vector Bernard Murray
Nru I digestion problems Bernard Murray
Superbroth for EVERYONE! sprbrth2.zip - sprbrth2.zip Bernard Murray
Help? Immunoprecipitation Bernard Murray
Plasmid Drawing Programme Bernard Murray
Time Resolved Fluorescence Europium Chelate Sources Wanted Tony Murray
Time Resolved Fluorescence Europium Chelate Sources Wanted Tony Murray
capillary PCR machine Tony Murray
STAINING MITOTIC STRUCTURES NAPOLI50
any Hpa2/MspI digest experts out there? How about StuI? Jon Nakamoto
electroporation of mammalian cells Olivier Namy
Research Scientists- Molecular Biology & Molecular Genetics Krishnan Nandabalan
western blot stripping protocol Dr. Kent L. Nastiuk
In situ hyb with Digoxigenin Dr. Kent L. Nastiuk
Differential Display Primers Viguerie Nathalie
isopropanol to precipitate DNA: how much? Viguerie Nathalie
Bad message with Ambion Message Machine Alan Neely
mMessage machine - Ambion Alan Neely
mMessage mcahine Ambion Alan Neely
RDA experience Ella Nemeth
western blot stripping protocol John A. Newitt
Do DNA DE-methylases exist? Brad Nicholson
cAMP-responsive luciferase constructs Jarmo Niemi
Panning with buffer insoluble peptide Eiji Nishiwaki
test Nokwanda.Makunga
antisense delivery by liposomes Pamela Norton
Western blot mystery Pamela Norton
Light Sensitivity of Tris Pamela Norton
blocking in northern blots Lauryl Maiya J Nutter
Need help with non-rad Southerns Lauryl Maiya J Nutter
Anyone successfully doing RNAse protection Assays? Lauryl Maiya J Nutter
probs digesting plant genomic Kirrily O'Connor
Ligase PCR Kevin O'Donnell
capillary sequencing Peadar O'Gaora
dna diffusion in agarose Bob Obar
DNA extraction of woody plant Kwang Oh
Primers storage.. George W Ohlandt
Integration efficiency: linear vs. circular DNA transfection. Namy Olivier
Using Biotin in ELISA's TA. Omiecinski
custom service: transgenic mices Myriam Onno
genetic evolutionary conservation Sandra Pena De Ortiz
Chromosomal localization Sandra Pena De Ortiz
Genetic Evolutionary conservation: Sandra Pena De Ortiz
Primers storage.. Osama
RT-PCR Osama
Protocol for Re-using of Qiagen tips Guy Oshiro
Chromosome-specific PCR Primers Anthony Otsuka
dut- ung- E. coli strain request Kevin G. Otto
WWW Medline Search? Francis Ouellette
Maleic acid buffer: Help me make it DOUGLAS PRASHER
Yellow Fluorescent Protein DOUGLAS PRASHER
Tandem repeats on end of gene? PRETORIUS
in situ hyb: eosinophilic cell-specific background Soon Paik
Biogel Dr Dave Parcej
peptide antigen success rate Mick Partis
Tet repressor antibodies??? Pramathesh S. Patel
Halls hyb? Christopher Coward Path
LM-PCR in vivo footprinting Cam Patterson
osteopontin Cam Patterson
Acridinium ester labelin Paul
Freshman chemistry Aaron Pawlyk
DH5a w/o plasmid growing on Amp? Karen Peck-Miller
Smear in gel lanes (mini preps) Chip Peele
Rapid Ligation Chip Peele
Lookig for antibody to guanylyl cyclase Simon Penson
"easy open" tubes Alan Pepper
Promoter elements Database???? Daniel Pereira
protein-protein interaction and expression libraries. Daniel Pereira
Protein turnover in Primary Cultures MJM. Perry
gt10 library problems Stephen Perry
southern (discomfort) Petur Henry Petersen
Southern blot : DIG or BIOTIN?? Petur Henry Petersen
Cloning a fragment from a gel Rodney Earl Pettway
Wrong buffer with restriction enzyme Rodney Earl Pettway
antibodies against beta-glucoronidase Matthias Pfeiffer
Can DTT be substituted by DTE ???? Stephen P. Driska PhD
QUANTITATIVE PCR Todd Miller - Pharmacology
One-Hybrid Andy Phillips
Trouble finding DNA concentration on Spec.. Andy Phillips
digital cameras Andy Phillips
Need help with non-rad Southerns Robert Pierce
Have you seen a CAT with wt p53 binding sequence? Robert Pierce
DNA extraction from formali-fixed tissues Landry Pierre-Alexandre
longevity of TAQ!! Problems with Pharmacia Landry Pierre-Alexandre
Smiles in agarose gels Landry Pierre-Alexandre
purifying short probes Eva Pietrzak
EtOH + PBS = precipitation? LU Pin
EtOH + PBS = precipitation? LU Pin
Mitochondrial DNA extraction? Kay A. Pleyte
Anyone have a Porcine OVARIAN cDNA library? David Plotner
Anyone have a Porcine OVARIAN cDNA library? David Plotner
Anyone have a Porcine OVARIAN cDNA library? David Plotner
Anyone have a Porcine OVARIAN cDNA library? David Plotner
Wanted: Porcine ovarian cDNA library David Plotner
Wanted: Porcine ovarian cDNA library David Plotner
BM plant DNA isolation kit Buffers? Oliver Politz
Potato tuber RNA Oliver Politz
Type of cytochrome Brent Pollock
Subcloning Blues Lena Polovnikova
Type of cytochrome Igor Polyakov
**ANYONE KNOW SOURCES OF THESE VECTORS?**" Andrei Popov
pipetman repair kit Jennifer L. Potter
Plant Biotech Workshop - update C. S. Prakash
Smear in gel lanes (mini preps) Kiley R. Prilliman
Smear in gel lanes (mini preps) Kiley R. Prilliman
cDNA synthesis from total RNA Kiley R. Prilliman
epitope tagging Kiley R. Prilliman
Sterilizing Hollow Fiber Bioreactor Cartridges Kiley R. Prilliman
Integration efficiency: linear vs. circular DNA transfection. Kiley R. Prilliman
probs digesting plant genomic QIAGEN
Problems with Qiagen Maxipreps? QIAGEN
S or SP sepharose differance? R.J.D.Reid
Transformation in CJ236 R.Visvanathan
Adenovirus expression systems RMMMS at CUNYVM.CUNY.EDU
Problems with Qiagen maxipreps? RMMMS at CUNYVM.CUNY.EDU
EtOH + PBS = precipitation? HIRANYA ROYCHOWDHURY
link two DNAs-a better idea needed RVDESHP0 at pwinet.upj.com
yeast cloning Ralle
Procaryotic promoter database? Pekka Rappu
Stripping of northern membranes Colin Rasmussen
bacterial housekeeping mRNA Colin Rasmussen
Sequencing gel question Colin Rasmussen
blunting with S1 nuclease Colin Rasmussen
Rapid Ligation Colin Rasmussen
Qiaex problems Colin Rasmussen
EtOH + PBS = precipitation? Colin Rasmussen
Automatic Film Developers? Colin Rasmussen
G418 Colin Rasmussen
EtOH + PBS = precipitation? Colin Rasmussen
Ligation problems on agarose-isolated fragments Colin Rasmussen
capillary PCR machine Randy Rasmussen
PVDF/phosphorimaging Frieda Reichsman
Help please: Sequence of pT7-5 Dr. Jutta Reidlinger
Electroporation of 3T3 fibroblasts Christian Reiser
Differential Display Kit Mary P. Remington
Genehunter Mary P. Remington
Restriction enzyme digestion problem S.L. Rennie
what's an easy way to look for cell death? Surgical Residence
Southern blot : DIG or BIOTIN?? Surgical Residence
PhastSystem/Western John H Richburg
PhastSystem/Western John H Richburg
FENTOMOLE PROTEIN SEQUENCING Richard Rickles
E. coli sec mutants AJ. Riley
Inclusion Bodies in E. coli S. Riley
RAS mutations Erwin Pauws & Carrie Ris
RT-PCR Erwin Pauws & Carrie Ris
DNA Isolation Kit Erwin Pauws & Carrie Ris
DdeI won't cut PCR product Erwin Pauws & Carrie Ris
osp--oligo selector program Bruce W. Ritchings
cDNA & long transcripts Karen Robb
LI-COR automatic sequencer? Rob Robson
How to modify a DNA construct? Richard Rohan
TMV Markus Rohrwild
RT-PCR vs. RNase Protection Assay - Detecting Sense and Antisense Don Rollins
BioConsumer Review? Louise Roloff
Freeze/Boil Cycling for Cell Lysis for rep-PCR Lemuel S. del Rosario
DNA fragmentation Wes Rose
Helper phage, help!! Marc Rosoff
Removing Phenol from Protein Fraction Eric Roush
Report centrifugal force in g units NOT rpm! (Rant) Robert Roxby
rat EGF antibodies? Wattiez Ruddy
Primer design software Gary Rudnick
Custum oligo on magnetic beads Heidi Ruijling
Vectorette PCR: Source for Vectorettes? Robert Rumpf
Tumor cell culture problems Schwartz jr. S.
(no subject) Mohamed SAAD
housekeeping gene rtpcr SEHuang
Northern witth oligos SEHuang
cosuppression vs. antisense constructs? SGASTW at ccmail.monsanto.com
Looking for information on OKT3 antibody CHIA BIN SIAK
Enzyme wanted - Cystathionine B synthase (EC 4.2.1.22) BRUCE SINCLAIR
unsubscribe SISTMANE at aol.com
Antisense vs. cosuppression constructs? SRBAER at ccmail.monsanto.com
Background in DGGE gels - who knows the cause? Igor R Sagdeev
Making cDNA library from Poly A minus RNA Michael Salvucci
TGGE vs DGGE Tony Sanchez
Isolation of RNA from wood Eric Sarnighausen
Silver staining problem Eric Sarnighausen
DH10B comp cells by the Inoue method Shoji Sawai
In situ hyb with Digoxigenin Karim Sayani
Square Phage plating plates. Greg Schaefer
Lambda DNA prep. Martin Schenker
Sequence of Lambda DASH II polylinker Martin Schenker
freezer purchase Fay Schmaltz
centrif. force: g or rpm? Denni Schnapp
selling of oneself Denni Schnapp
MJ Tetrad thermocycler Steve Schramm
home-made ECL solutions? Norbert Schuelke
Phenol/Chloroform extraction--physical basis? Christopher Schultz
rat embryo fibroblast source Ralph Schuster
Differential display kit: who is selling it? Ralph Schuster
dna diffusion in agarose Ralph Schuster
dna diffusion in agarose Ralph Schuster
dna diffusion in agarose Ralph Schuster
Taq error R.K. Scopes
bacculovirus expression Ian Sealy
Exponentially growing U937 ?? Dirk Seegert
plant-TF primers Georg J. Seifert
in situ hyb: eosinophilic cell-specific background Mike Serfas
Do DNA DE-methylases exist? Q. Shang
extracting RNA from bone Sue Shapses
Silver staining problem Joel Sheffield
Platinum wire for electrophoresis in France Joan Shields
Promoter elements Database???? Benny Shomer
Primer design software Benny Shomer
promoter sequences Benny Shomer
PCR Primers Database - UPDATE. Benny Shomer
Smear in gel lanes (mini preps) Song-Kun Shyue
Protocol for Re-using of Qiagen tips Song-Kun Shyue
PSG5vector Song-Kun Shyue
LDLR deficient cell line? Hiljar Sibul
Plasmid request Igor Sidorenkov
IGF Type 1 receptor cDNA Alan Sielicki
Helper phage, help!! Nathan Siemers
Maxiprep woes Mike Sklar
mung bean nuclease Chris Skory
Restriction enzyme digestion problem Robert Slany
mRNA isolation in E. coli Robert Slany
Do NTP's precipitate with ethanol? How efficiently? Robert Slany
Double Stranded Mutagenesis-Chameleon Leslie Sloan
Lysing difficult "Bugs" Koen De Smet
DdeI won't cut PCR product Koen De Smet
RNA Band problem Stephanie Smith
Designing primers Haunani A. Soares
Platinum wire for electrophoresis in France Sophie Launey, These Andre Gerard 31 12 96, Ifremer DRV RA, 46.36.30.07
staining of phosphoproteins Keld Sorensen
Supplier for secondary antibody goat anti-mouse Keld Sorensen
Bioline Keld Sorensen
BioConsumer Review? Keld Sorensen
detergent assay Keld Sorensen
Oligo 4.0 sofware. Help Giorgio Spagnol
Sulfate uptake inhibitor: Help Giorgio Spagnol
NATIVE GEL ELECTROPHORESYS ANSWER Giorgio Spagnol
(no subject) Giorgio Spagnol
RABBIT anti-mouse IgM Giorgio Spagnol
Advice on genomic DNA preps wanted Cathy Sprankle
mycoplasma Anne Spurkland
beta trypsin Suneal Sreedharan
PCR from blood samples John E. Stacy
RDA of cDNA Peter Staeheli
mycoplasma Guillaume Stahl
Qiaex problems John Stebbins
PCR, Tm, and ligations..? John Stebbins
Sequencing Kit for IR-ATP-Labeling? Mueller Steffen
LI-COR automatic sequencer? Herta Steinkellner
Automatic Film Developers? Dag Stenberg
PCR on bryophytes Hans Stenoien
FW: Freeze/Boil Cycling for Cell Lysis for rep-PCR Stewart, Robert
FW: PCR contaminant with abs peak 299 Stewart, Robert
Immonoprecipitation problem S Stray
Address of Novagene ? Ulrich Strych
Ligation problems on agarose-isolated fragments Dr.Sailesh Surapureddi
Smear in gel lanes (mini preps) Dr.Sailesh Surapureddi
library amplification Dr.Sailesh Surapureddi
How much bis-acrylamide in aa-gel Dr.Sailesh Surapureddi
Qiaex problems Dr.Sailesh Surapureddi
Smiles in agarose gels Dr.Sailesh Surapureddi
Ethidium Bromide Dr.Sailesh Surapureddi
Looking for Protein Sequencing Service Company Dr.Sailesh Surapureddi
A novice Q in competent cell preparation. Sailesh Surapureddi
Protein Crystallization Supplier R. Bryan Sutton
ADH assay R. Bryan Sutton
Need rat TNF-R antibody R. Bryan Sutton
Fill-in reaction R. Bryan Sutton
Protein w/ no Trp quantitation: help!!! R. Bryan Sutton
purchasing power supply for sequencing Michael Szardenings
Ligation problems on agarose-isolated fragments Michael Szardenings
Needed: mammalian expr. vector with NcoI Michael Szardenings
Double Labeling Problem Michael Szardenings
Low stringency Southern trouble KELLY THOME
SENSE vs. ANTI-SENSE DNA VOTING RESULTS THORN at LAN.TJHSST.EDU
Transformation/electroporation of phytoplankton TLANE at watson.princeton.edu
DNA analysis software TUCKER at AFP76.HUMC.EDU
ADH assay Paul Tadich
ADH assay Paul Tadich
staining of phosphoproteins Ron Tate
DTT Stability Ron Tate
PhastSystem/Western Ron Tate
PhastSystem/Western Ron Tate
Removing Phenol from Protein Fraction Ron Tate
resolution on SDS-PAGE Ron Tate
resolution on SDS-PAGE Ron Tate
Can seq gel sit O/N before drying? Danielle Teninges
Annealing temperature of 10-mer oligo Terence
Differential Display Tommy the Terrorist
[Q] How to measure Itraconazole in serum? I. Thalmann
Silver staining problem Tom Thatcher
mycoplasma Kelly J. Thibault
Nitrocellulose Phage Lifts-- Reprobing? Michael W. Thompson
Mung Bean Nuclease Maria Thuveson
Mapping random mutagenesis Greg Tobin
Plasmid prep of yeast two hybrid library? None Today
Maxiprep woes Michael Tomasson
SENSE vs. ANTI-SENSE DNA CALL FOR VOTES Anthony Tomlinson
NEED PCR primer program for MAC Anthony Tomlinson
polishing PCR fragments with T4 Pol ? Anthony Tomlinson
Reactivation of DFP inhibited serine-esterase Achim Treumann
Protein dimerisation in SDS-gel Vadim Tsvetnitsky
anti-HA mAb 12CA5 Joseph Tumang
Qiaex problems Simon Twigger
Search for universal bacterial 5S primer Shaun Tyler
Lysing difficult "Bugs" Shaun Tyler
Making cDNA library from Poly A minus RNA Shaun Tyler
DNA Isolation Kit Shaun Tyler
PCR templates on Automated DNA sequencers Shaun Tyler
capillary sequencing Shaun Tyler
Annealing temperature of 10-mer oligo Shaun Tyler
(no subject) UZS13B at ibm.rhrz.uni-bonn.de
probs digesting plant genomic Paul E. Ulanch
Virus dilutions and RT-PCR Tom Unruh
RABBIT anti-mouse IgM Virginia Urquidi
anti-cyclin A antibody wanted Michael Vagell
Wanted: anti-cyclin A antibody for western blot Michael Vagell
Anti-cyclin A antibody Michael Vagell
Lox and Cre plasmid Edgar Valencia
Help PCR product ligation problem Edgar Valencia
Expression Vectors (fwd) Edgar Valencia
G418 Patrick Van
NATIVE GEL ELECTROPHORESYS ANSWER Margon Vandongen
Qiaex problems Margon Vandongen
Ligation problems on agarose-isolated fragments Margon Vandongen
Gel analysis using the GelCompar program Mario Vaneechoutte
Glycerol inhibition of PCR? Mario Vaneechoutte
Search for universal bacterial 5S primer Mario Vaneechoutte
Freeze/Boil Cycling for Cell Lysis for rep-PCR Mario Vaneechoutte
Can seq gel sit O/N before drying? Tom Vasicek
want comments on phosphorimagers: Molec.Dynamics vs BioRad Tom Vasicek
Stripping of northern membranes Olle Vidal
digital cameras Sigurdur E. Vilhelmsson
PCR Frustrations Tom Vink
5' biotinylation of RNA Tom Vink
bacculovirus expression Marc Visconti
tagged protein expression Jim Voeller
Partial RE digestion - please advise! Jim Voeller
epitope tagging Jim Voeller
Partial RE digestion - please advise! Jim Voeller
Qiaex problems Jim Voeller
Differential Display Jim Voeller
Temperatursensitivity of Firefly-Luciferase Matthias Vogel
Help! Reusing Qiagen-tip Matthias Vogel
Half-life of GFP in vivo? Matthias Vogel
PCR trouble!!! Bjoern Voldborg
Chromosomal location of muscarinic receptor genes Amy Voltz
FIAU & Gancyclovir Amy Voltz
Deletion dilemma WILLIS at BCRSSU.AGR.CA
where is Culture Tek maker of VacuuPette WSchick at aol.com
Fluorometer: to buy or not? WSchick at aol.com
Time Resolved Fluorescence Europium Chelate Sources Wanted WSchick at aol.com
protocal for staining isozymes WSchick at aol.com
(no subject) WSchick at aol.com
Smiles in agarose gels WSchick at aol.com
Fwd: Biotech Recruiting Site WSchick at aol.com
Desperately seeking mini "Euro" tissue grinders WSchick at aol.com
Scanning densitometry problem WSchick at aol.com
Smiles in agarose gels WSchick at aol.com
Biogel WSchick at aol.com
g/ rpm, pH+temp, V+cm.,conductivity of buffers WSchick at aol.com
Biogel WSchick at aol.com
TGGE vs DGGE &2D DGGE WSchick at aol.com
Superbroth for EVERYONE! (NOT) Mark Walberg
Nick translation after random PCR of paraffin DNA Fred Waldman
aminosuberate and aminodecanoate Jonathan Walton
source of aminosuberate and aminodecanoate? Jonathan Walton
aminosuberate and aminodecanoate source Jonathan Walton
aminosuberate and aminodecanoate source Jonathan Walton
more about mung bean nuclease Edward Wang
PCR, Tm, and ligations..? Edward Wang
Differential Display Edward Wang
RNA isolation Edward Wang
GFP truncation Peter Wang
Lambda YES vector Peter Wang
Do DNA DE-methylases exist? Peter Wang
Custum oligo on magnetic beads Peter Wang
Mapping random mutagenesis Peter Wang
How to covalently link DNA to beads? Peter Wang
Heteroduplex Mobility Assay Peter Wang
Anti-Mycoplasma agents Glenn A. Warr
Helper phage, help!! Vitor Warwar
Epitope Tagging Suggestions Wanted A. John Watson
Northern witth oligos A. John Watson
Cutting PCR-created restriction sites A. John Watson
Cryomarkers - where can i buy them?? A. John Watson
Custum oligo on magnetic beads Dr Mark Watson
**ANYONE KNOW LOCATIONS OF THESE CELL LINES*** Dr Mark Watson
**ANYONE KNOW SOURCES OF THESE VECTORS?** Dr Mark Watson
FIAU source wanted Georg Weitzer
FIAU source wanted Georg Weitzer
FIAU source wanted Georg Weitzer
FIAU source wanted Georg Weitzer
FIAU source wanted Georg Weitzer
FIAU source wanted Georg Weitzer
bacterial housekeeping mRNA Jim Wellehan
competent bacteria Gregg B Wells
blocking in northern blots CK Wen
blocking in northern blots CK Wen
blocking in northern blots CK Wen
Smear in gel lanes (mini preps) CK Wen
Isolation of RNA from wood CK Wen
probs digesting plant genomic CK Wen
Inclusion Bodies in E. coli CK Wen
Inclusion Bodies in E. coli CK Wen
Non-radioactive end-labelling CK Wen
Desalting column CK Wen
Trouble finding DNA concentration on Spec.. CK Wen
Trouble finding DNA concentration on Spec.. CK Wen
Do NTP's precipitate with ethanol? How efficiently? CK Wen
mRNA isolation in E. coli Chi-Kuang Wen
cDNA synthesis from total RNA Chi-Kuang Wen
Potato tuber RNA Chi-Kuang Wen
CNBr activated affinity supports? Geoffrey D. Wheelock
Gelshift: bands w/o extract? Jennifer Wiedenman
ATP replacement Will
Trouble finding DNA concentration on Spec.. William B. Melchior, Jr.
isopropanol to precipitate DNA: how much? Ann Williams
Suggestions - PCR from Phage Isolates Tori D. Williams
Hello All: Les Willis
Microtitre plates: edge effects? Iain Wilson
Texas Red Rat Antibody Mr D.J.A. Wilson
PCR trouble!!! Troels Wind
Monitoring RNA synthesis Neil Winegarden
Monitoring RNA synthesis Neil Winegarden
Lambda Screening Problems Harry J. Witchel
Supplier for secondary antibody goat anti-mouse Harry J. Witchel
Qiaex problems Arthur Wohlwill
Alkaline gels-Better method? David T Wong
pipetman repair kit Donald Chun Kit Wong
Microtitre plates: edge effects? Donald Chun Kit Wong
RNA from mouse skin. Michael Wong
Bioline Stuart Wood
c-fos and c-jun expression Jim Woodgett
resolution on SDS-PAGE R. Woodward
Exon trapping Public Workstation
Exon trapping Public Workstation
glyoxalation of RNA Russell L Wrobel
isopropanol to precipitate DNA: how much? Zhengyang Wu
Help with He sparging of HPLC solvents Philip J. Wyatt
E.coli genomic library help wanted XIAOMING YANG
E.coli genomic library help wanted XIAOMING YANG
Dear Sir: XIAOMING YANG
E.coli. genomic library help wanted XIAOMING YANG
Biogel Michael Yacuboski
Left DNA out at RT...advice? Mayumi Yagi
CEA scFv help!!! Yu Yanxing
test Fei Ye
Apyrase - concentrations, storage Ian A. York
Tet-repressible genes - timing Ian A. York
Library kits Ian A. York
Delta Fingerprinting David Tak Yan Yu
Cymbus Biosciences, Ltd. address ZEROSIGMA
Scaling down DNA synthesis on ABI 381 A ? Peter Zickert
polishing PCR fragments with T4 Pol ? Frances Hannan Zoology
Suggestions - PCR from Phage Isolates Frances Hannan Zoology
lipid phase transition temperature edwin alcantara
QUANTITATIVE PCR alena at scientia.up.ac.za
Boiling miniprep problems with DH5-alpha gordon allison
Helper phage, help!! gordon allison
Smear in gel lanes (mini preps) gordon allison
mRNA isolation in E. coli gordon allison
HELP please: Inverse PCR with genomic fragments! gordon allison
PCR trouble!!! gordon allison
Removing Phenol from Protein Fraction gordon allison
RT-PCR gordon allison
Genomic S. blot problems gordon allison
test anonymous
(none) mark band
Colorimetric assay for alkaline phosphatase (SEAP) bbraun at ucla.edu
Clontech "PromoterFinder" experiences? bbraun at ucla.edu
Wrong buffer with restriction enzyme bbraun at ucla.edu
RDA of cDNA bbraun at ucla.edu
100 bp molecular weight marker bbraun at ucla.edu
Rapid Ligation bbraun at ucla.edu
luciferase vectors bbraun at ucla.edu
Mapping random mutagenesis bbraun at ucla.edu
Differential Display bbraun at ucla.edu
Differential Display bbraun at ucla.edu
Differential Display bbraun at ucla.edu
protein-protein interaction and expression libraries. biol.ruu.nl at N.P.Dantuma
(no subject) boyce at CSHL.ORG
"Stratacooler" sources? john brennand
cDNA synthesis from total RNA john brennand
Lambda DNA prep. john brennand
Wrong buffer with restriction enzyme john brennand
Differential display kit: who is selling it? john brennand
Address of Novagene ? john brennand
RNA hydrolysis john brennand
(REQUEST) who has a probe for c-fos? john brennand
100 bp molecular weight marker john brennand
DH5a in DMEM john brennand
anti-Tat antibody john brennand
Molecular Probes' address. john brennand
"easy open" tubes john brennand
DE52 Lambda Phage DNA prep john brennand
Making cDNA library from Poly A minus RNA john brennand
**ANYONE KNOW LOCATIONS OF THESE CELL LINES*** john brennand
Desperately seeking mini "Euro" tissue grinders john brennand
Expression vector containing SV40 large T antigen? john brennand
Integration efficiency: linear vs. circular DNA transfection. john brennand
(no subject) john brennand
Mung Bean Nuclease brett
Subcloning Blues brett
Ethidium bromide and clothes brett
DTT Stability brett
Blatant Selling of Myself! brett
Differential Display brett
Help? Immunoprecipitation brett
Using Biotin in ELISA's phillip buckhaults
lamba gem12 cloning kit c.hoege
Halls hyb? tom chappell
EGF receptor ab charest
E. coli Promoters sean colloms
superbroth protocol finally finished davesmith at bioch.tamu.edu
superbroth protocol--OVERWHELMED! - suprbrth.zip (0/8) davesmith at bioch.tamu.edu
superbroth protocol--OVERWHELMED! - suprbrth.zip (1/8) davesmith at bioch.tamu.edu
superbroth protocol--OVERWHELMED! - suprbrth.zip (2/8) davesmith at bioch.tamu.edu
superbroth protocol--OVERWHELMED! - suprbrth.zip (3/8) davesmith at bioch.tamu.edu
superbroth protocol--OVERWHELMED! - suprbrth.zip (4/8) davesmith at bioch.tamu.edu
superbroth protocol--OVERWHELMED! - suprbrth.zip (5/8) davesmith at bioch.tamu.edu
superbroth protocol--OVERWHELMED! - suprbrth.zip (6/8) davesmith at bioch.tamu.edu
superbroth protocol--OVERWHELMED! - suprbrth.zip (7/8) davesmith at bioch.tamu.edu
superbroth protocol--OVERWHELMED! - suprbrth.zip (8/8) davesmith at bioch.tamu.edu
REPOSTING SUPRBRTH (fewer parts) - suprbrth.zip (0/1) davesmith at bioch.tamu.edu
REPOSTING SUPRBRTH (fewer parts) - suprbrth.zip (1/1) davesmith at bioch.tamu.edu
What format is the Superbroth file? davesmith at bioch.tamu.edu
Superbroth for EVERYONE! sprbrth2.zip - sprbrth2.zip (0/1) davesmith at bioch.tamu.edu
Superbroth for EVERYONE! sprbrth2.zip - sprbrth2.zip (1/1) davesmith at bioch.tamu.edu
Help with T7 detection davesmith at bioch.tamu.edu
Superbroth for EVERYONE! (was NOT, but now IS) davesmith at bioch.tamu.edu
blunting with S1 nuclease rlab davis
Electrocompetent Cells simon day
DNA Synthesizer info wanted dnalab
Hypoxia chamber? ege
Postdoc Salaries? ege
Bafilomycin A1 elena
protein expression in mammalian cells. errabolu
purification of 140 Kbp from agarose erty
Fluorometer for RNA???? fisher at radonc.unc.edu
(REQUEST) who has a probe for c-fos? fisher at radonc.unc.edu
PCR smear futers at biovax.leeds.ac.uk
Can one gene be used to select for OR against.... g1687jkarh at umbsky.cc.umb.edu
decapping RNA gabriel,abram
decapping RNA gabriel,abram
Heteroduplex Mobility Assay gbbmv
Anyone successfully doing RNAse protection Assays? genes at unixg.ubc.ca
Sterilizing Hollow Fiber Bioreactor Cartridges gustilo at mail.med.upenn.edu
Superbroth for EVERYONE! (NOT) gustilo at mail.med.upenn.edu
Superbroth for EVERYONE! (NOT) gustilo at mail.med.upenn.edu
osp--oligo selector program h.woo
Pre-hybridization not required for in situ hybridization? h.woo
Primase activity assay xin hang
Differential Display Primers hcslab2 at postbox.acs.ohio-state.edu
Use of GeneScreen Plus for Southerns hcslab2 at postbox.acs.ohio-state.edu
anti CAT antibody??? helix.nih.gov
Molecular Probes' address. helix.nih.gov
luciferase vectors helix.nih.gov
Cre recombinase hraapg at marc.cri.nz
Looking for Sequencing Service Company hshibat at ibm.net
Non-radioactive end-labelling sabah hussain
Monovalent streptavidin hx41 at CITYSCAPE.CO.UK
Tet-repressible genes - timing iddavis at vms.cis.pitt.edu
Molecular Probes' address. Administrador de iihema.fmed.uba.ar
Gelshift: bands w/o extract? ijiwaru at wheel.dcn.davis.ca.us
qieex problems j.r.stephen
Can DTT be substituted by DTE ???? janke
Northern blot and NybondN+membrane janke
link two DNAs-a better idea needed jay
Overbaked Northern blots jfh
Chromosomal localization jfh
housekeeping gene rtpcr jms93
Sulfate uptake inhibitor: Help jms93
Qiaex problems jms93
RT-PCR jms93
Buffer PN jms93
Immunoprecipitation using proteinA agarose kim myung jong
test kim myung jong
Help? Immunoprecipitation kim myung jong
Anaerobic jar supplier jost at vetsci.microvet.edu.au
MacVector-type program for IBM??? jovermeyer at shrsys.hslc.org
Problems with Qiagen Maxipreps? jovermeyer at shrsys.hslc.org
inter alu pcr jprice at MGRP.BGSM.EDU
promoter sequences kalch
promoter sequences kalch
Transgenic rat cooperation kang at msvax.mssm.edu
Insolubility in pTrxFus Expression System. Help!! James kling
southern (discomfort) kramerse at wmvx.lvs.dupont.com
multiple m-RNA measurement lars.warngard
Sources of Anti-LexA john lewis
(no subject) lgbrieba
PCR problems lgbrieba
PSG5vector liz_caetta at stratagene.com
PSG5vector liz_caetta at stratagene.com
Protein gel lmrc.
protein gels lmrc.
protein gels lmrc.
Supplier for secondary antibody goat anti-mouse logan
Program. mutlichan. pump logan
Method for protein extraction from root tissue logan
Maleic acid buffer (aside) logan
Help! removal of rRNA from bulk RNASKIP/NEWSGROUP ltxnei01 at uctvms.uct.ac.za
Ganciclovir and HeLa cells? michelle malott
Ganciclovir and HeLa Cells - help! michelle malott
Ganciclovir and HeLa Cells - help! michelle malott
Fluorometer: to buy or not? mamstutz at postbox.acs.ohio-state.edu
Expression vector containing SV40 large T antigen? mamstutz at postbox.acs.ohio-state.edu
Sequencing gel question jose manaligod
Plasmid Preparation Rolf marteijn
Superbroth for EVERYONE! sprbrth2.zip - sprbrth2.zip (0/1) glover martin
Smiles in agarose gels marty
Maxiprep woes matt_ambros at hhs.dresden.k12.nh.us
Method for protein extraction from root tissue mauricio at CNPMS.EMBRAPA.BR
Stable dilution of peroxidase mbro at seqnet.dl.ac.uk
Looking for Protein Sequencing Service Company mcmreddy
klappts? melkony at wwupop.uni-muenster.de
Doktorand gesucht melkony at wwupop.uni-muenster.de
Mab against soluable Fibrin mentrup at uni-muenster.de
Hydropathy plot program mikel at vaxc.cc.monash.edu.au
Representational Difference Analysis mikel at vaxc.cc.monash.edu.au
NATIVE GEL ELECTROPHORESYS ANSWER ming at msvax.mssm.edu
Protein w/ no Trp quantitation: help!!! ming at msvax.mssm.edu
Method for protein extraction from root tissue ming at msvax.mssm.edu
Postdoc Salaries? ming at msvax.mssm.edu
100 bp molecular weight marker mobio at electriciti.com
Does Anybody Have An Answer negar moshiri
c-fos and c-jun expression negar moshiri
immunoprecipitations in thymocytes.... mrtour at stud.med.cornell.edu
Digestion of Core Protein nc26 at wwa.com
Digestion of Core Protein nc26 at wwa.com
monoclonal GPC-HPLC separation nc26 at wwa.com
Sources of baculovirus expression systems nswema05 at angis.su.OZ.AU
MACROPHAGE ELECTROPORATION o0c9869 at tam2000.tamu.edu
Freshman chemistry oRutebuka
pipetman repair kit john ohlsson
TnphoA Mutagenesis orbashu at ubvms.cc.buffalo.edu
HELP on reporter gene assay otello at sc2a.unige.ch
HELP: AADA assay otello at sc2a.unige.ch
Group I phospholipase A2 pa691004 at express.ssctr.bcm.tmc.edu
DNA Isolation Kit june eva paciga
Yellow Fluorescent Protein chip peele
DH5a w/o plasmid growing on Amp? chip peele
pH Meter perkitm at WKUVX1.WKU.EDU
Left DNA out at RT...advice? a.a. peters
RNA as blocking agent in PC pgegen at rnaworld.bio.ukans.edu
Alternative Methods for Preparing Lifts pmmcnutt at phoenix.princeton.edu
Wizard maxipreps ppldrb.nott.ac.uk at
Protocol for Re-using of Qiagen tips pw15 at cornell.edu
melkony@wwupop.uni-muenster.de quidde at uni-muenster.de
Isolation of DNA from mammalian tissue rlee
E-C Apparatus Corp? rvosatka at opal.tufts.edu
Biotinylating Peptides s.mclaren
non radioactiv cAMP measurement schille at uni-muenster.de
arrested nascent chains silverback
Subcloning Blues trey simmons
Saccaromyces cerevisiae trey simmons
Blocking Phosphotyrosine Blots sam simons
Blocking Phosphotyrosine Blots sam simons
Address of Novagene ?? sam simons
Cutting PCR-created restriction sites sam simons
Sources of baculovirus expression systems sam simons
Desalting column skim at ALLIANT.SNU.AC.KR
NEED PCR primer program for MAC slee at opal.tufts.edu
RNA extraction using Catrimox soc at uk.ac.aber
Help with T7 detection ssieben at imb-jena.de
Thioredoxin fusion kit? stanley.liu at amgen.com
extracting dna from agarose (re: which kit...) jane staunton
Can seq gel sit O/N before drying? cynthia stryker
housekeeping gene rtpcr suihuang at usa1.com
Summary: Protein dimerisation in SDS gels sunmail
untranslated region syamada at sb.gunma-u.ac.jp
Plasmid isolation syeh at BIOLOGY.WATSTAR.UWATERLOO.CA
Need information about 2 expression vectors Manfred Maier t2539
Subcloning Blues tera
dut- ung- E. coli strain request tera
None toxic buffer and dye for electrophores thanh at wwg3.uovs.ac.za
Freeze/Boil Cycling for Cell Lysis for rep-PCR tromick at class.org
PCR Frustrations upyers
Help PCR product ligation problem upyers
PCR primer software upyers
problems with RNAs denatured with Glyoxal(in Northerns) upyers
purifying short probes upyers
Heteroduplex Mobility Assay upyers
HELP please: Inverse PCR with genomic fragments! user
specific pe9600 protocol varyc.mmcri at mmc.org
anti-Tat antibody varyc.mmcri at mmc.org
PMSF timing varyc.mmcri at mmc.org
capillary PCR machine vcameron at chmeds.ac.nz
influence of ethanol verlooy at uia.ua.ac.be
nicking DNA plasmids on purpose vipond at bsa.bris.ac.uk
freeze-squeeze vsood at acs.ucalgary.ca
200 bp pcr product ruth e.w. walker
Northern blot and NybondN+membrane wellman
Alternative Methods for Preparing Lifts pete wilson
library amplification wvcrieki at lmb1.rug.ac.be
alu pcr xxx at bgsm.edu
help with "PNA", a DNA with peptide xzluo at cc.memphis.edu
Need Fv fragment to P-Tyr KIm doo yeon
Good inhibitors to the CIP KIm doo yeon
Nuclear run-on assays yu

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Archived on: Sun Mar 6 20:57:28 EST 2005

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