hot start beads
horton at biosci.cbs.umn.edu
Tue Jan 2 15:16:58 EST 1996
PRASHERD at DELPHI.COM wrote:
: We have used Invitrogen's HotStart Beads with wonderful results
: but they are VERY EXPENSIVE. Cost is a major concern to us
We use vaseline petroleum jelly instead of wax, and dispense it with
a syringe. See:
Horton, R.M., Hoppe, B.L., and Conti-Tronconi, B.M. AmpliGrease: "Hot-start"
PCR using petroleum jelly. BioTechniques 16(1):42-43, 1994.
This paper also incluses our protocol for small (10ul) hot-start reactions.
I am maintaining a FAQ page for the "AmpliGrease" method on my web site
at http://188.8.131.52. Comments and questions are welcome.
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