resuspending intractable DNA
cwen at aux.btny.purdue.edu
Tue Jan 2 12:47:23 EST 1996
sullivan at gwis2.circ.gwu.edu (Steven Sullivan) wrote:
>I prepped some genomic DNA using a guanidinium-based kit by Pharmacia, and
>the resulting pellets refuse to go into solution (distilled water). Since
>Pharmacia's closed until Tuesday, I wondered if anyone here has hints on
>resuspending tough DNA pellets. I already tried heating/boiling.
What type of tissue did you use for DNA preparation? I always notice
that the pellet does not go to solution if the polysaccharides content
is too high.
More information about the Methods