resuspending intractable DNA

CK Wen cwen at aux.btny.purdue.edu
Tue Jan 2 12:47:23 EST 1996


Sullivan wrote:
sullivan at gwis2.circ.gwu.edu (Steven Sullivan) wrote:
>
>
>I prepped some genomic DNA using a guanidinium-based kit by Pharmacia, and
>the resulting pellets refuse to go into solution (distilled water).  Since
>Pharmacia's closed until Tuesday, I wondered if anyone here has hints on
>resuspending tough DNA pellets.  I already tried heating/boiling. 
>

What type of tissue did you use for DNA preparation?  I always notice
that the pellet does not go to solution if the polysaccharides content
is too high. 

Chi-Kuang





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