rt-pcr problems

Belinda J Wagner bwagner at bgsm.edu
Tue Jan 2 11:45:50 EST 1996

First off, the optimal annealing temp of a PCR primer is usually way 
above the highest reaction temp for RT.  I've had the best results using 
Superscript RT II from Gibco/BRL and either oligo-dT or random primers.

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