pH of TE

Jose M. Bautista jbautist at hgmp.mrc.ac.uk
Tue Jan 2 09:34:43 EST 1996



On Tue, 2 Jan 1996 you at somehost.somedomain wrote:

> Someone in the lab has told me that you don't need to pH TE for DNA work
> but the pH of his TE is about 10, will this affect the DNA and any
> subsequent manipulations i.e. enzyme digestion or PCR?
> 
> 
Yes, it can affect. 
pH of TE must be 7.5-8.0
pH it, your DNA will be more happy there

Jose M. Bautista
RPMS-London




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