Looking 4 lon- E. Coli

Mikhail Alexeyev malexeyev at biost1.thi.tmc.edu
Wed Jan 3 17:40:13 EST 1996

In article <4cerdi$d03 at netserv.unmc.edu>, mhorn at netserv.unmc.edu (Mark
Horn) wrote:

> Hello netters-
>     Im looking from protease - E Coli. cells. More specifically
> Im looking for lon - cells (for example SG1611). I am looking
> to use these cells for routine fusion protein expression.
> Any input that you may have on this subject or strains that
> you would be able to provide would be appreciated.

All E. coli B strains are naturally lon- (check ATCC). You may also
consider BL21 strain that is   lon-   (BL21 you can get from many
companies Novagen, Stratagene, etc.).

Good luck.

M. Alexeyev

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