Question on how to radio-label a protein.

Mark Gosink GOSINK at MACC.WISC.EDU
Thu Jan 4 17:34:56 EST 1996


Dear Netters:
 
	Can anyone point me towards references for radio-labelling proteins
(antibodies) other than iodination? I need to do some experiments in another
lab and they are not set up to handle 125-I.
 
	I have tried immunoblotting with either an alkaline-phosphatase
NBT/BCIP or a HR peroxidase/chemiluminecent detection scheme; however, these
were not sensitive enough.
 
	Thanks in advance for any suggestions.
 
*************************************************************************
* Dr. Mark Gosink			Post-doctoral Fellow		*
* Department of Horticulture		(608) 262-1622	voice   	*
* University of Wisconsin-Madison	(608) 262-4743	FAX     	*
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* Madison ,WI 53706			GOSINK at MACC.WISC.EDU (internet) *
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