pH of TE

Dominique nil
Thu Jan 4 06:49:57 EST 1996

> Someone in the lab has told me that you don't need to pH TE for DNA work
> but the pH of his TE is about 10, will this affect the DNA and any
> subsequent manipulations i.e. enzyme digestion or PCR?
.Why don't you prepare TE using stock solution of Tris HCl pH7.5-8 and
EDTA stock solution (pH 8). 
It works, and besides normal conc of Tris is 10mM and EDTA is 1mM in TE
and thus with stock solutions is easy to prepare.

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