Ferland Louis H.
ferlandl at ERE.UMontreal.CA
Sat Jan 6 01:18:30 EST 1996
On Fri, 5 Jan 1996, Richard Moldwin wrote:
> Date: Fri, 5 Jan 1996 14:34:44 GMT
> From: Richard Moldwin <rmoldwin at midway.uchicago.edu>
> To: methods at net.bio.net
> Subject: Re: Sense/Antisense debate
> This sense/antisense stuff is making my head spin. When
> we first started designing antisense oligos, we had the
> same problems of conflicting usages in the literature.
> Can't we abandon the sense/coding terminology completely?
> How about:
> For DNA:
> Beta Actin Transcribed Strand
> Beta Actin Non-transcribed Strand
> (The gene name is important in case the opposite strand is
> also transcribed)
> For mRNA:
> Beta Actin mRNA
> cDNA to Beta Actin mRNA
> Beta Actin cDNA oligo
> (The latter would be the same as the confusing beta actin
> I assume that there is a consensus that cDNA means a copy
> of DNA complementary to the mRNA.
All this stands fine with me, except we have to account for RNA that are
other than mRNA, but that's easy: rRNA, tRNA, etc. The "cRNA" term
required, for example, in some quantitative RT-PCR procedures (RNA
complementary to a usually mutated cDNA) will continue to be a mind bender,
but I think it still can fly.
Dr. Louis H. Ferland
Centre de Recherche, Hotel-Dieu de Montreal
Dept de Nutrition, Universite de Montreal
Phone: (514) 843-2757 FAX: (514) 843-2719
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