Troubles with Pfu polymerase
Holger at gem.univie.ac.at
Fri Jan 5 10:48:29 EST 1996
In article <4cjekp$ll0 at saphir.jouy.inra.fr>, hatzfeld at msdos.ensam.inra.fr
(Yves hatzfeld) wrote:
> Hi Netters!
> I have troubles with amplifying a 1.5 kb fragment with pfu polymerase
> When I do the same experiments using Taq instead, I haven't any problem
> and I obtain the expected products ...
> So why the Hell it does not work?
> Any idea would be very wellcome, before I encounter a nervous
It´s always the same story with pfu polymerase.
try 100 microliter reaction volume not 50
increase the primer concentration, 500 ng each
I don´t know why but it is like it is
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