More and more confused about making SENSE

Ferland Louis H. ferlandl at ERE.UMontreal.CA
Sat Jan 6 03:55:49 EST 1996

On 5 Jan 1996, Erik A. Williams wrote:

> Date: 5 Jan 1996 11:36:01 -0800
> From: Erik A. Williams <eaw at ACSU.BUFFALO.EDU>
> To: methods at
> Subject: More and more confused about making SENSE
> Hey Netters,
> I've been trying to follow the SENSE/ANTISENSE thread, and thought I 
> understood it at one point, but thanks to Dr. Lasky's very confusing 
> explanations, I'm really confused now.  Can someone else besides Dr. 
> Lasky try to explain this to me?
> My understanding has alsways been consistent with what Mick Jones writes:
> >5'-AAGCTTCTACTGAGT-3'  DNA strand 1
> >3'-TTCGAAGATGACTCA-5'  DNA strand 2
> >         |
> >         |
> >         V
> >5'-AAGCUUCUACUGAGU-3'  RNA strand 3
> >
> >In my book the following nomenclature seems okay;
> >
> >Strand 1 = SENSE strand (because it is the same sequence as the 
> >transcribed RNA [strand 3])
> >Strand 2 = ANTISENSE strand (this strand can bind to in vivo transcribed 
> >RNA, form a duplex and inhibit gene expression, i.e. Antisense therapy).
> >
> >In the old days Strand 2 was called the CODING strand because it was the DNA
> >strand that was the
> >template used to make the transcribed RNA (strand 3), and hence strand 1 was
> which seems to be in disagreement with what Dr. Lasky writes, although I 
> may be reading him wrong.  This is how I learned it, and to me this is
> what makes sense (sorry, bad pun).

In which textbook? It is not how *I* learned it. I agree that it does make 
sense (same bad pun), but so does the other possibility, as I posted 
earlier. The bottom line (not strand, OOPS! another bad pun) is 
CONFUSION. About the Lasky comment, I think this was HIS bailout.

> Erik

Dr. Louis H. Ferland
Centre de Recherche, Hotel-Dieu de Montreal
Dept de Nutrition, Universite de Montreal
Phone: (514) 843-2757     FAX: (514) 843-2719

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