M13RF DNA..gDNA contamination

Duncan Cochran cochran at biosci.uq.oz.au
Mon Jan 8 01:04:42 EST 1996


Hi

Have been trying to purify midi and mini scale M13RF DNA from dut- ung-
strain for past few weeks.  Tried a few different alkaline lysis
procedures, different strains, shortened lysis time and different
amounts of cells in lysis.  Can't get rid of the genomic DNA
contamination, no matter how gentle the lysis.  

Before I start to use cesium gradients to make M13RF (which will be a
big drag since I am making many individual mutants) I was hoping one of
you out there could assist.

Thanks

Duncan Cochran



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