fixation-sensitive antibody

username at bay.cc.kcl.ac.uk username at bay.cc.kcl.ac.uk
Mon Jan 8 12:09:46 EST 1996


Can anybody suggest other fixation procedures for an
antibody sensitive to crosslinking methods such as
paraformaldehyde? At the moment, methanol-based fixes
seem to be ok but in whole-mounts this causes embryos
to collapse in upon themselves ?stopping antibody
penetrating. Any ideas much appreciated.

Chris



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