[HELP]Cloning double stranded oligo DNA fragment into vector.

Michael Daws Michael.Daws at ucmp.umu.se
Mon Jan 8 09:21:26 EST 1996


I've never done this before, so maybe I am overlooking something, but it 
would appear that step2 would just ligate your fragment together into 
big circles!  The other thing I would suggest is to use a longer 
incubation time for your ligation.  Also I find that a 1:1 molar ratio 
of fragment to plasmid gives the best results although other people may 
disagree with this one.

Mike Daws





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