[HELP]Cloning double stranded oligo DNA fragment into vector.
Michael.Daws at ucmp.umu.se
Mon Jan 8 09:21:55 EST 1996
I've never done this before, so maybe I am overlooking something, but it
would appear that step2 would just ligate your fragment together into
big circles! The other thing I would suggest is to use a longer
incubation time for your ligation. Also I find that a 1:1 molar ratio
of fragment to plasmid gives the best results although other people may
disagree with this one.
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